Matsuda M, Aoki N, Kawaoi A
Kidney Int. 1979 Jun;15(6):612-7. doi: 10.1038/ki.1979.80.
By the indirect immunofluorescent and immunoenzymatic techniques with monospecific antiserum against urinary procoagulant (a tissue factor which accelerates blood coagulation), we found the urinary procoagulant in the kidney distributed to the loop of Henle and distal convoluted tubules. In these areas urinary procoagulant was found in association with the luminal and intercellular borders as well as in the cytoplasm of epithelial cells. Both the descending and ascending limbs of Henle were equally stained. The cytoplasmic staining was patchy in distribution among cells of distal tubules and was predominantly localized in the supranuclear areas. Glomeruli, the proximal tubular cells, the vascular wall, and the interstitium were not stained. There was, however, fluorescent staining along the epithelial layers of the Bowman's capsule, which was observed only in the frozen sections. Casts in the distal tubules were also positively stained. These findings suggest that urinary procoagulant is synthesized in the epithelial cells of these particular parts of nephron and is secreted into urine, although its physiologic roles and pathologic significance are not entirely known.
通过使用针对尿促凝剂（一种加速血液凝固的组织因子）的单特异性抗血清的间接免疫荧光和免疫酶技术，我们发现肾脏中的尿促凝剂分布于亨氏袢和远曲小管。在这些区域，尿促凝剂存在于管腔和细胞间边界以及上皮细胞的细胞质中。亨氏袢的降支和升支染色程度相同。远曲小管细胞间的细胞质染色呈斑片状分布，主要定位于核上区域。肾小球、近端肾小管细胞、血管壁和间质均未染色。然而，仅在冰冻切片中观察到鲍曼囊上皮层有荧光染色。远曲小管中的管型也呈阳性染色。这些发现表明，尿促凝剂是在肾单位这些特定部位的上皮细胞中合成并分泌到尿液中的，尽管其生理作用和病理意义尚不完全清楚。
