Lwaleed B A, Chisholm M, Francis J L
University Department of Haematology, Southampton University Hospitals, UK.
J Clin Pathol. 1999 Mar;52(3):219-24. doi: 10.1136/jcp.52.3.219.
Activation of blood coagulation is a common complication of cancer and inflammation in both humans and experimental animals. Increased production of tissue factor--the principal initiator of the coagulation process--by endothelial cells, monocytes, and macrophages has been implicated in these conditions.
To investigate whether urinary tissue factor (uTF) might reflect the state of monocyte/macrophage activation and be a useful diagnostic test.
Urine was centrifuged at 51,000 g to sediment tissue factor containing membrane vesicles. The tissue factor was then solubilised in beta-octyl-glucopyranoside and assayed in a specific chromogenic assay adapted for use in microtitre plates.
The assay proved to be sensitive, specific, and reproducible. The normal range of uTF was relatively narrow and unaffected by age, sex, or cigarette smoking. Levels were not significantly influenced by storage of urine samples before assay or by the presence of fresh blood in the urine sample.
This method may have diagnostic application in the study of haemostatic activation in patients with cancer and other disease states.
血液凝固的激活是人类和实验动物癌症及炎症常见的并发症。内皮细胞、单核细胞和巨噬细胞中组织因子（凝血过程的主要启动因子）产量增加与这些情况有关。
研究尿组织因子（uTF）是否能反映单核细胞/巨噬细胞的激活状态并成为一项有用的诊断检测方法。
尿液在51,000g下离心，使含组织因子的膜泡沉淀。然后将组织因子溶解于β-辛基葡萄糖苷中，并在适用于微量滴定板的特定显色测定法中进行检测。
该检测方法被证明具有敏感性、特异性和可重复性。uTF的正常范围相对较窄，不受年龄、性别或吸烟的影响。检测前尿液样本的储存或尿液样本中新鲜血液的存在对其水平没有显著影响。
该方法可能在癌症及其他疾病状态患者止血激活的研究中具有诊断应用价值。
