Neurovascular Research Laboratory, Vall d'Hebron Institute of Research (VHIR)-Universitat Autónoma de Barcelona, Barcelona, Spain.
Stroke Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.
J Stroke Cerebrovasc Dis. 2024 Jan;33(1):107467. doi: 10.1016/j.jstrokecerebrovasdis.2023.107467. Epub 2023 Nov 8.
To understand the similarities and differences between acute ischemic stroke and acute myocardial infarction (AMI) to help in the development of specific or common treatment strategies.
Using an aptamer-based proteomic array, we measured and compared 1310 circulating proteins in the blood of 40 patients with AIS, 9 patients with AMI, and 31 healthy controls. Pathway enrichment analysis was performed using GSEA and g:profiler.
Ninety-four proteins were differentially expressed in AIS, and 284 were differentially expressed in AMI. Of these, 8 were specific to cerebral ischemia, and 197 were specific to myocardial infarction. Forty-two proteins were altered in both ischemia processes. Most altered pathways in AIS could be classified as immune response, cell cycle processing, molecular transport, or signaling. Pathways altered in AMI were mostly related to lipid metabolism and transport, highlighting cholesterol metabolic processes and estrogen signaling. In both types of ischemia, we found pathways related to metabolism, specifically purine metabolism, and signaling processes, such as TNF signaling or MAPK1/3.
The present study revealed proteins and pathways that were specifically altered in cerebral ischemia, in cardiac ischemia, or in both diseases, providing information on the similarities and differences of ischemic conditions. The role of common and specific proteins and pathways should be explored in detail to find possible therapeutic targets.
了解急性缺血性脑卒中（AIS）与急性心肌梗死（AMI）之间的异同，有助于制定特定或通用的治疗策略。
我们使用基于适体的蛋白质组芯片，测量并比较了 40 例 AIS 患者、9 例 AMI 患者和 31 名健康对照者血液中的 1310 种循环蛋白。使用 GSEA 和 g:profiler 进行通路富集分析。
AIS 中有 94 种蛋白差异表达，AMI 中有 284 种蛋白差异表达。其中，8 种蛋白是脑缺血特异性的，197 种蛋白是心肌梗死特异性的。在这两个缺血过程中，有 42 种蛋白发生了改变。AIS 中改变最明显的通路可分为免疫反应、细胞周期处理、分子转运或信号转导。AMI 中改变的通路主要与脂质代谢和转运有关，突出了胆固醇代谢过程和雌激素信号转导。在这两种类型的缺血中，我们发现了与代谢相关的通路，特别是嘌呤代谢，以及 TNF 信号或 MAPK1/3 等信号转导过程。
本研究揭示了在脑缺血、心肌缺血或两种疾病中特异性改变的蛋白和通路，为缺血性疾病的相似性和差异性提供了信息。应详细探讨共同和特异蛋白及通路的作用，以寻找可能的治疗靶点。
