Mori R
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Nov;38(11):1982-8.
It is not so difficult to operate on the hydrosalpinx with macrosurgical or microsurgical methods, but difficult to initiate pregnancy after the operation because little is known about the post-operative condition of the hydrosalpinx. In this study time sequential observations of the growing experimental hydrosalpinx with the two hemoclip method was performed during morphological change using the microscope, sequential electron microscope and transmission electron microscope with cationized ferritin as an ultrastructural marker. The following results were obtained: Size of hydrosalpinx: Until 15 weeks after the operation, the hemo-clipping hydrosalpinx became longer but thereafter there was no change. The maximum diameter of the hydrosalinx became wider until 20 weeks after treatment. Peristaltic of hydrosalpinx: Tubal peristaltics were observed until 7 weeks after treatment, but not thereafter. SEM findings: Decreases in the amount of cilia started 1 week after treatment, and 7 weeks after treatment partial excoriations of epithelium were observed. 20 weeks after treatment excoriations of epithelium were observed widely. A decrease in the amount of cilia was observed but no loss was observed. The negative charges on the tubal endometrium due to TEM using cationized ferritin: The negative charges on the tubal endometrium were decreasing both on the secretory cells and ciliary cells.
采用宏观手术或显微手术方法对输卵管积水进行手术操作并非难事，但术后难以受孕，因为对输卵管积水术后状况了解甚少。在本研究中，使用显微镜、连续电子显微镜和透射电子显微镜，以阳离子铁蛋白作为超微结构标记物，在形态学变化过程中，采用双夹法对实验性输卵管积水的生长进行了时间序列观察。获得了以下结果：输卵管积水的大小：术后15周内，夹闭血管后的输卵管积水变长，但此后无变化。治疗后20周内，输卵管积水的最大直径变宽。输卵管积水的蠕动：治疗后7周内观察到输卵管蠕动，但此后未观察到。扫描电子显微镜结果：治疗后1周开始观察到纤毛数量减少，治疗后7周观察到上皮部分剥脱。治疗后20周广泛观察到上皮剥脱。观察到纤毛数量减少，但未观察到纤毛缺失。使用阳离子铁蛋白的透射电子显微镜观察输卵管内膜的负电荷：输卵管内膜分泌细胞和纤毛细胞上的负电荷均在减少。
