College of Animal Sciences, Zhejiang University, Hangzhou, China.
Agricultural Technology Extension Center of Zhejiang Province, Hangzhou, China.
Pestic Biochem Physiol. 2023 Nov;196:105610. doi: 10.1016/j.pestbp.2023.105610. Epub 2023 Sep 2.
The widespread use of pesticides hampers the immune system of non-target organisms, however, there is a lack of common biomarkers to detect such effects. Myeloid differentiation primary response factor 88 (MyD88) is a crucial junction protein in the Toll-like receptor signaling pathway, which plays an important role in the inflammatory response. In this study, we investigated MyD88 as a potential biomarker for pesticide-induced stress. Phylogenetic analysis revealed that MyD88 was a conserved protein in the evolution of vertebrates and invertebrates. MyD88s usually have death domain (DD) and Toll/interleukin-1 receptor (TIR) domain. Bombyx mori (B. mori) is an important economic insect that is sensitive to toxic substances. We found microbial pesticides enhanced the expression level of MyD88 in B. mori. Transcriptome analysis demonstrated that MyD88 expression level was increased in the fatbody after dinotefuran exposure, a third-generation neonicotinoid pesticide. Moreover, the expression of MyD88 was upregulated in fatbody and midgut by imidacloprid, a first-generation neonicotinoid pesticide. Additionally, insect growth regulator (IGR) pesticides, such as methoprene and fenoxycarb, could induce MyD88 expression in the fatbody of B. mori. These results indicated that MyD88 is a potential biomarker for pesticide-induced stress in B. mori. This study provides novel insights into screening common biomarkers for multiple pesticide stresses and important implications for the development of more sustainable pest management strategies.
农药的广泛使用会损害非靶标生物的免疫系统，但目前缺乏用于检测此类影响的通用生物标志物。髓样分化初级反应因子 88（MyD88）是 Toll 样受体信号通路中的关键连接蛋白，在炎症反应中发挥重要作用。在这项研究中，我们研究了 MyD88 作为一种用于检测农药诱导应激的潜在生物标志物。系统发育分析表明，MyD88 是脊椎动物和无脊椎动物进化过程中的一种保守蛋白。MyD88 通常具有死亡结构域（DD）和 Toll/白细胞介素-1 受体（TIR）结构域。家蚕是一种重要的经济昆虫，对有毒物质敏感。我们发现微生物农药能增强家蚕中 MyD88 的表达水平。转录组分析表明，在三嗪类新烟碱类农药啶虫脒暴露后，家蚕脂肪体中的 MyD88 表达水平增加。此外，第一类新烟碱类农药吡虫啉也能上调脂肪体和中肠中的 MyD88 表达。另外，昆虫生长调节剂（IGR）类农药，如灭幼脲和苯氧威，也能诱导家蚕脂肪体中的 MyD88 表达。这些结果表明，MyD88 是家蚕中农药诱导应激的潜在生物标志物。本研究为筛选多种农药应激的通用生物标志物提供了新的思路，并对开发更可持续的害虫管理策略具有重要意义。
