College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Environ Pollut. 2022 Aug 15;307:119562. doi: 10.1016/j.envpol.2022.119562. Epub 2022 May 31.
Dinotefuran is a third-generation neonicotinoid pesticide and is increasingly used in agricultural production, which has adverse effects on nontarget organisms. However, the research on the impact of dinotefuran on nontarget organisms is still limited. Here the toxic effects of dinotefuran on an important economic species and a model lepidopteran insect, Bombyx mori, were investigated. Exposure to different doses of dinotefuran caused physiological disorders or death. Cytochrome P450, glutathione S-transferase, carboxylesterase, and UDP glycosyl-transferase activities were induced in the fat body at early stages after dinotefuran exposure. By contrast, only glutathione S-transferase activity was increased in the midgut. To overcome the lack of sensitivity of the biological assays at the individual organism level, RNA sequencing was performed to measure differential expressions of mRNA from silkworm larvae after dinotefuran exposure. Differential gene expression profiling revealed that various detoxification enzyme genes were significantly increased after dinotefuran exposure, which was consistent with the upregulation of the detoxifying enzyme. The global transcriptional pattern showed that the physiological responses induced by dinotefuran toxicity involved multiple cellular processes, including energy metabolism, oxidative stress, detoxification, and other fundamental physiological processes. Many metabolism processes, such as carbon metabolism, fatty acid biosynthesis, pyruvate metabolism, and the citrate cycle, were partially repressed in the midgut or fat body. Furthermore, dinotefuran significantly activated the MAPK/CREB, CncC/Keap1, PI3K/Akt, and Toll/IMD pathways. The links between physiological, biochemical toxicity and comparative transcriptomic analysis facilitated the systematic understanding of the integrated biological toxicity of dinotefuran. This study provides a holistic view of the toxicity and detoxification metabolism of dinotefuran in silkworm and other organisms.
呋虫胺是一种第三代新烟碱类杀虫剂，在农业生产中被越来越多地使用，对非靶标生物有不良影响。然而，关于呋虫胺对非靶标生物的影响的研究仍然有限。本研究调查了呋虫胺对一种重要经济物种和一种模式鳞翅目昆虫家蚕的毒性作用。暴露于不同剂量的呋虫胺会导致生理紊乱或死亡。在暴露于呋虫胺后早期，脂肪体中的细胞色素 P450、谷胱甘肽 S-转移酶、羧酸酯酶和 UDP 糖基转移酶活性被诱导。相比之下，仅中肠中的谷胱甘肽 S-转移酶活性增加。为了克服个体水平生物测定的灵敏度不足，本研究通过 RNA 测序测量了暴露于呋虫胺后家蚕幼虫 mRNA 的差异表达。差异基因表达谱分析显示，暴露于呋虫胺后，各种解毒酶基因显著增加，与解毒酶的上调一致。全局转录谱表明，呋虫胺毒性诱导的生理反应涉及多个细胞过程，包括能量代谢、氧化应激、解毒和其他基本生理过程。许多代谢过程，如碳代谢、脂肪酸生物合成、丙酮酸代谢和柠檬酸循环，在中肠或脂肪体中部分受到抑制。此外，呋虫胺显著激活了 MAPK/CREB、CncC/Keap1、PI3K/Akt 和 Toll/IMD 途径。生理、生化毒性与比较转录组分析之间的联系促进了对呋虫胺综合生物毒性的系统理解。本研究为家蚕和其他生物中呋虫胺的毒性和解毒代谢提供了一个整体的观点。
