School of Chemistry and Chemical Engineering, Guangdong Provincial Engineering Research Center for Green Fine Chemicals, South China University of Technology, Guangzhou 510641, China.
Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
ACS Appl Mater Interfaces. 2023 Nov 22;15(46):53871-53880. doi: 10.1021/acsami.3c14479. Epub 2023 Nov 9.
integration of enzymes with covalent organic frameworks (COFs) to form hybrid biocatalysts is both significant and challenging. In this study, we present an innovative strategy employing deep eutectic solvents (DESs) to synergistically synthesize COFs and shield cytochrome c (Cyt c). By utilizing DESs as reaction solvents in combination with water, we successfully achieved rapid and encapsulation of Cyt c within COFs (specifically COF-TAPT-TFB) under ambient conditions. The resulting Cyt c@COF-TAPT-TFB composite demonstrates a remarkable preservation of enzymatic activity. This encapsulation strategy also imparts exceptional resistance to organic solvents and exhibits impressive recycling stability. Additionally, the enhanced catalytic efficiency of Cyt c@COF-TAPT-TFB in a photoenzymatic cascade reaction is also showcased.
将酶与共价有机框架（COFs）集成形成杂化生物催化剂既具有重要意义，又极具挑战性。在这项研究中，我们提出了一种创新策略，利用深共熔溶剂（DESs）协同合成 COFs 并保护细胞色素 c（Cyt c）。我们使用 DESs 作为反应溶剂，与水结合，在环境条件下成功地实现了 Cyt c 在 COFs（特别是 COF-TAPT-TFB）中的快速封装。所得的 Cyt c@COF-TAPT-TFB 复合材料表现出显著的酶活性保留。这种封装策略还赋予了它对有机溶剂的优异抗性，并表现出令人印象深刻的循环稳定性。此外，Cyt c@COF-TAPT-TFB 在光酶级联反应中的增强催化效率也得到了展示。
