McClure N C, Venables W A
J Gen Microbiol. 1986 Aug;132(8):2209-18. doi: 10.1099/00221287-132-8-2209.
Pseudomonas putida mt-2 (ATCC 33015) carrying the TOL plasmid pWW0 could adapt to growth on the aromatic amines aniline and m- and p-toluidine. In strain UCC2, a derivative adapted to rapid growth on these compounds, they were oxidatively deaminated to catechol or 4-methylcatechol, which in turn were dissimilated by a meta-cleavage pathway. The aniline/toluidine oxygenase and the meta-cleavage pathway enzymes were inducible by aromatic amines. Evidence is presented that in strain UCC2, plasmid pWW0 has undergone deletion of its catabolic genes, and that it is a novel plasmid, pTDN1, which is involved in the catabolism of aniline and m- and p-toluidine. The meta-cleavage pathway genes which are carried by pTDN1 were shown not to have originated in pWW0.
携带TOL质粒pWW0的恶臭假单胞菌mt-2（ATCC 33015）能够适应在芳香胺苯胺、间甲苯胺和对甲苯胺上生长。在UCC2菌株中，该菌株是适应于在这些化合物上快速生长的衍生物，这些芳香胺被氧化脱氨生成儿茶酚或4-甲基儿茶酚，而儿茶酚或4-甲基儿茶酚又通过间位裂解途径被异化。苯胺/甲苯胺加氧酶和间位裂解途径的酶可被芳香胺诱导。有证据表明，在UCC2菌株中，质粒pWW0已发生分解代谢基因的缺失，并且它是一种新型质粒pTDN1，该质粒参与苯胺、间甲苯胺和对甲苯胺的分解代谢。pTDN1携带的间位裂解途径基因并非起源于pWW0。
