伯克霍尔德氏菌属菌株AK-5中对氨基苯酚的代谢途径与苯胺及带有C-4取代基的苯胺的代谢途径不同。
The metabolic pathway of 4-aminophenol in Burkholderia sp. strain AK-5 differs from that of aniline and aniline with C-4 substituents.
作者信息
Takenaka Shinji, Okugawa Susumu, Kadowaki Maho, Murakami Shuichiro, Aoki Kenji
机构信息
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokko, Kobe, Japan.
出版信息
Appl Environ Microbiol. 2003 Sep;69(9):5410-3. doi: 10.1128/AEM.69.9.5410-5413.2003.
Abstract
Burkholderia sp. strain AK-5 utilized 4-aminophenol as the sole carbon, nitrogen, and energy source. A pathway for the metabolism of 4-aminophenol in strain AK-5 was proposed based on the identification of three key metabolites by gas chromatography-mass spectrometry analysis. Strain AK-5 converted 4-aminophenol to 1,2,4-trihydroxybenzene via 1,4-benzenediol. 1,2,4-Trihydroxybenzene 1,2-dioxygenase cleaved the benzene ring of 1,2,4-trihydroxybenzene to form maleylacetic acid. The enzyme showed a high dioxygenase activity only for 1,2,4-trihydroxybenzene, with K(m) and V(max) values of 9.6 micro M and 6.8 micro mol min(-1) mg of protein(-1), respectively.
摘要
伯克霍尔德氏菌属菌株AK-5利用4-氨基苯酚作为唯一的碳、氮和能源。基于气相色谱-质谱分析鉴定出的三种关键代谢物，提出了菌株AK-5中4-氨基苯酚的代谢途径。菌株AK-5通过1,4-苯二酚将4-氨基苯酚转化为1,2,4-三羟基苯。1,2,4-三羟基苯1,2-双加氧酶裂解1,2,4-三羟基苯的苯环形成马来酰乙酸。该酶仅对1,2,4-三羟基苯表现出高双加氧酶活性，其米氏常数（K(m)）和最大反应速度（V(max)）值分别为9.6微摩尔和6.8微摩尔每分钟每毫克蛋白质。
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