Van Dongen W M, De Graaf F K
J Gen Microbiol. 1986 Aug;132(8):2225-34. doi: 10.1099/00221287-132-8-2225.
Recombinant plasmids encoding a Vibrio cholerae haemagglutinin were isolated from the highly virulent V. cholerae strain C5 by cosmid cloning. Both Escherichia coli HB101 containing the recombinant plasmids and V. cholerae C5 were able to agglutinate a variety of erythrocytes from human and animal origin; this haemagglutination was not inhibited by D-mannose or L-fucose. Subcloning of the recombinant cosmid DNA revealed that a 1.3 kb DNA fragment was sufficient for haemagglutinin production in E. coli HB101. Under direction of this 1.3 kb Vibrio DNA fragment, two proteins were made in E. coli minicells, of 27 and 10 kDa. Haemagglutinin-encoding sequences were not detected in every V. cholerae strain.
通过黏粒克隆从高毒力霍乱弧菌菌株C5中分离出编码霍乱弧菌血凝素的重组质粒。含有重组质粒的大肠杆菌HB101和霍乱弧菌C5均能够凝集多种人和动物来源的红细胞；这种血凝反应不受D-甘露糖或L-岩藻糖的抑制。重组黏粒DNA的亚克隆显示，一个1.3 kb的DNA片段足以在大肠杆菌HB101中产生血凝素。在这个1.3 kb霍乱弧菌DNA片段的指导下，大肠杆菌微小细胞中产生了两种蛋白质，分子量分别为27 kDa和10 kDa。并非在每个霍乱弧菌菌株中都检测到血凝素编码序列。
