Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France.
Fish Physiol Biochem. 2023 Dec;49(6):1381-1390. doi: 10.1007/s10695-023-01262-w. Epub 2023 Nov 10.
This study presents a novel non-lethal sampling method for assessing fatty acid (FA) composition in juvenile European sea bass (Dicentrarchus labrax) using subcutaneous white muscle biopsies. This research aimed to evaluate the suitability of the biopsy for FA analysis using two lipid extraction protocols and comparing them to a lethal routine method. The results showed that a mass of fresh tissue as low as 1.4 mg provided good quality FA chromatograms for both reserve and membrane lipids. Although the biopsy method displayed high variability in terms of FA quantity among intra-individual replicates, it showed good FA profile repeatability in both reserve and membrane lipids. The study highlights the potential of this non-lethal approach for studying FA dynamics in fish, with its application being particularly promising for ecological and experimental studies. However, careful biopsy implementation is recommended to account for potential lipid droplet and lipid distribution variability within the tissue.
本研究提出了一种新颖的非致死性采样方法，用于评估幼年欧洲鲈鱼（Dicentrarchus labrax）的脂肪酸（FA）组成，方法是使用皮下白肌活检。本研究旨在通过两种脂质提取方案评估活检用于 FA 分析的适宜性，并将其与致死性常规方法进行比较。结果表明，低至 1.4 毫克的新鲜组织质量就可以为储备和膜脂提供良好的 FA 色谱图。尽管活检方法在个体内重复的 FA 数量方面表现出高度的变异性，但它在储备和膜脂中的 FA 谱重复性都很好。该研究强调了这种非致死性方法在鱼类 FA 动态研究中的潜力，其应用对于生态和实验研究尤其有前景。然而，建议谨慎实施活检，以考虑组织内脂质滴和脂质分布的潜在变异性。
