过量表达和 RNA 干扰转基因毛状根的转录组分析揭示了其在大豆抗胞囊线虫中的潜在负作用。

Transcriptome Analysis of Overexpressing and RNA-Interferencing Transgenic Hairy Roots Reveals Underlying Negative Role in Soybean Resistance to Cyst Nematode.

机构信息

Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China.

College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.

出版信息

J Agric Food Chem. 2023 Nov 22;71(46):18059-18073. doi: 10.1021/acs.jafc.3c05617. Epub 2023 Nov 10.

DOI:10.1021/acs.jafc.3c05617

PMID:37948664

Abstract

Ubiquitination genes are key components of plant responses to biotic stress. , a ubiquitination gene, plays a negative role in soybean resistance to soybean cyst nematode (SCN). In this study, we employed high-throughput sequencing to investigate transcriptional changes in overexpressing and RNA-interfering transgenic hairy roots. Totally, 7661 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that DEGs were significantly enriched in disease resistance and signal transduction pathways. In addition, silencing and by siRNA, the total number of nematodes was decreased by 33.48% and 27.47% than control plants, respectively. Further, GUS activity and reactive oxygen species (ROS) assays revealed that , , and respond to SCN parasitism and interfere with the accumulation of ROS in plant roots, respectively. Collectively, our study provides insights into the molecular mechanism of in soybean resistance to SCN.

摘要

泛素化基因是植物响应生物胁迫的关键组成部分。泛素化基因在大豆对大豆胞囊线虫（SCN）的抗性中发挥负调控作用。在本研究中，我们采用高通量测序技术研究了泛素化基因过表达和 RNAi 转基因毛状根中的转录组变化。总共鉴定出 7661 个差异表达基因（DEGs）。GO 富集和 KEGG 通路分析表明，DEGs 显著富集在抗病和信号转导途径中。此外，通过 siRNA 沉默和，与对照植株相比，线虫总数分别减少了 33.48%和 27.47%。进一步的 GUS 活性和活性氧（ROS）检测表明，、和对 SCN 寄生做出响应，并分别干扰植物根中 ROS 的积累。总之，本研究为泛素化基因在大豆抗 SCN 中的分子机制提供了新的见解。