用于肺转移研究的小鼠原位单肺移植方案。
Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies.
机构信息
Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany; Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Department of Thoracic Surgery, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg 79106, Germany.
出版信息
STAR Protoc. 2023 Dec 15;4(4):102701. doi: 10.1016/j.xpro.2023.102701. Epub 2023 Nov 10.
The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023)..
移植模型提供了机会，可以使用相应的基因修饰供体动物来评估感兴趣的分子对于肿瘤细胞外渗的相关性。在这里，我们提出了一种在小鼠中进行原位单肺移植的方案，作为肺转移研究的工具。我们描述了动物准备、肺移植和肿瘤细胞注射的步骤。然后，我们详细介绍了在基因修饰的左肺和未修饰的右肺实质之间直接比较肿瘤细胞扩散的程序。有关此方案的使用和执行的完整详细信息，请参见 Giannou 等人（2023 年）。
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