Zaun Gregor, Liu Siyang, Webendörfer Maximilian, Wani Abubakar, Rodriguez Diego, Green Douglas R, Kalkavan Halime
Department of Medical Oncology, West German Cancer Center, University Hospital Essen, University Duisburg-Essen, 45122 Essen, Germany; Medical Faculty, University Duisburg-Essen, Essen, Germany.
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
STAR Protoc. 2025 Apr 23;6(2):103793. doi: 10.1016/j.xpro.2025.103793.
Murine orthotopic tumor models can accurately resemble cancer biology characteristics including metastasis, drug sensitivity, and remodeling of the tumor microenvironment. Here, we present a protocol for establishing murine orthotopic lung tumors and assessing contralateral pulmonary metastasis by flow cytometry. We describe steps for marking tumor cells with label retention dyes, preparing cell mixtures in Matrigel, and performing intrapulmonary injection. We then detail procedures for ex vivo processing of lungs followed by fluorescence-activated cell sorting (FACS) data analysis and quantification of metastatic capacity. For complete details on the use and execution of this protocol, please refer to Kalkavan et al..
小鼠原位肿瘤模型能够准确模拟癌症生物学特征，包括转移、药物敏感性以及肿瘤微环境的重塑。在此，我们展示了一种建立小鼠原位肺肿瘤并通过流式细胞术评估对侧肺转移的方案。我们描述了用标记保留染料标记肿瘤细胞、在基质胶中制备细胞混合物以及进行肺内注射的步骤。然后，我们详细说明了肺的体外处理程序，随后进行荧光激活细胞分选（FACS）数据分析和转移能力的量化。有关此方案的使用和执行的完整详细信息，请参考卡尔卡万等人的研究。
