S-甲基-L-半胱氨酸亚砜作为蜂王浆的特征性标志物：非靶向和靶向代谢组学分析的见解。
S-methyl-L-cysteine sulfoxide as a characteristic marker for rape royal jelly: Insights from untargeted and targeted metabolomic analysis.
机构信息
State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Key Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture and Rural Affairs, Beijing 100093, China.
State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Key Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture and Rural Affairs, Beijing 100093, China; Fujian Agriculture and Forestry University, Fuzhou City 350002, China.
出版信息
Food Chem. 2024 Mar 30;437(Pt 1):137880. doi: 10.1016/j.foodchem.2023.137880. Epub 2023 Oct 26.
Among the varieties of royal jelly (RJ), often referred to as "liquid gold", rape royal jelly (RRJ) is popular because of its superior nutritional value. However, existing physicochemical indicators fall short in identifying different types of RJ. Utilizing a UPLC-Q-Exactive Orbitrap-MS technique combined with metabolomics, this study was the first to identify S-methyl-L-cysteine sulfoxide (SMCSO) in RRJ, thereby it from other types of RJ. Subsequent to this observation, a method based on UPLC-QqQ-MS/MS, was developed and optimized for precise SMCSO quantification in RRJ, achieving a detection range of 77.55-112.68 mg/kg. Furthermore, an analysis of honey and bee bread harvested from the same batch of rape plants confirmed the presence of SMCSO, with the highest concentration detected in rape bee bread. In light of these findings, SMCSO emerges as a potent authenticity marker for RRJ.
在蜂王浆（RJ）的各种品种中，常被称为“液体黄金”的油菜蜂王浆（RRJ）因其卓越的营养价值而广受欢迎。然而，现有的理化指标在识别不同类型的 RJ 时存在不足。本研究首次利用 UPLC-Q-Exactive Orbitrap-MS 技术结合代谢组学方法，在 RRJ 中鉴定出 S-甲基-L-半胱氨酸亚砜（SMCSO），从而将其与其他类型的 RJ 区分开来。在此观察结果的基础上，开发并优化了基于 UPLC-QqQ-MS/MS 的 RRJ 中 SMCSO 的精确定量方法，检测范围为 77.55-112.68mg/kg。此外，对同一批油菜植物收获的蜂蜜和蜂粮进行分析，证实了 SMCSO 的存在，在油菜蜂粮中检测到的浓度最高。有鉴于此，SMCSO 成为 RRJ 的一种强有力的真实性标志物。
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