Department of Molecular Genetics and Cancer, Nitte University Centre for Science Education and Research (NUCSER), Nitte (Deemed to be University), Kotekar-Beeri Road, Deralakatte, Mangalore 575018, India.
Department of Food Safety and Nutrition, Nitte University Centre for Science Education and Research (NUCSER), Nitte (Deemed to be University), Mangalore 575018, India.
Biomed Pharmacother. 2023 Dec 31;169:115854. doi: 10.1016/j.biopha.2023.115854. Epub 2023 Nov 9.
Garlic (Allium sativum) is an important flavouring component in Indian cuisine. Allicin, a sulphur containing compound, is the most abundant component of garlic and has been widely studied for its antimicrobial and antioxidant properties. It is also known to play a role in the regulation of blood pressure and cholesterol levels. Despite the known health benefits associated with allicin, systematic studies on its anti-cancer properties using animal models are very limited. This study aimed to develop a simple method for the extraction of allicin from fresh garlic, study the stability of the extracted compound at various temperatures, and evaluate the antioxidant, anti-proliferative, pro-apoptotic and anti-angiogenic properties in zebrafish. A five-month stability study indicated that allicin remains significantly stable at temperatures 4 °C and below but shows extensive degradation if stored at room temperature. The in vivo studies in zebrafish using a combination of mutants and transgenic lines demonstrated the antioxidant, anti-proliferative, apoptotic and anti-angiogenic properties of allicin. The study highlights the importance of natural bioactive compounds as potential anti-cancer agents that can be studied further.
大蒜（Allium sativum）是印度烹饪中一种重要的调味料。大蒜素是一种含硫化合物，是大蒜中最丰富的成分，因其具有抗菌和抗氧化特性而被广泛研究。它还被认为在调节血压和胆固醇水平方面发挥作用。尽管大蒜素具有已知的健康益处，但使用动物模型对其抗癌特性进行系统研究非常有限。本研究旨在开发一种从新鲜大蒜中提取大蒜素的简单方法，研究在不同温度下提取化合物的稳定性，并在斑马鱼中评估其抗氧化、抗增殖、促凋亡和抗血管生成特性。为期五个月的稳定性研究表明，大蒜素在 4°C 及以下温度下保持显著稳定，但如果储存在室温下则会大量降解。使用突变体和转基因系的斑马鱼体内研究表明，大蒜素具有抗氧化、抗增殖、凋亡和抗血管生成特性。这项研究强调了天然生物活性化合物作为潜在抗癌药物的重要性，这些药物可以进一步研究。
