Wade W G, Aldred M J, Walker D M
J Med Microbiol. 1986 Dec;22(4):319-23. doi: 10.1099/00222615-22-4-319.
A medium based upon tryptone, yeast extract, cystine (TYC) agar and incorporating bacitracin and sucrose has been evaluated for selective isolation of Streptococcus mutans. The effect of varying the concentrations of sucrose and bacitracin on the recovery of two standard strains was investigated. Growth of S. mutans NCTC 10449 was significantly inhibited by increasing concentrations of sucrose but was not affected by bacitracin; the reverse was seen with S. sobrinus strain 6715. The best compromise between recovery of the streptococci and growth of other organisms was obtained with a final sucrose concentration of 20% and bacitracin 0.2 units/ml. In comparison with three other selective media, this medium gave the highest recovery rate of standard strains, indicating that it is superior to mitis-salivarius bacitracin (MSB) agar for the recovery of S. mutans from saliva.
一种基于胰蛋白胨、酵母提取物、胱氨酸（TYC）琼脂并加入杆菌肽和蔗糖的培养基已被评估用于变形链球菌的选择性分离。研究了改变蔗糖和杆菌肽浓度对两种标准菌株回收率的影响。变形链球菌NCTC 10449的生长随着蔗糖浓度的增加而受到显著抑制，但不受杆菌肽影响；而远缘链球菌6715菌株的情况则相反。最终蔗糖浓度为20%且杆菌肽为0.2单位/毫升时，在链球菌回收率和其他微生物生长之间取得了最佳平衡。与其他三种选择性培养基相比，该培养基对标准菌株的回收率最高，表明在从唾液中回收变形链球菌方面，它优于轻唾杆菌肽（MSB）琼脂。
