致龋菌的评估

Evaluation of cariogenic bacteria.

作者信息

Nishikawara Fusao, Nomura Yoshiaki, Imai Susumu, Senda Akira, Hanada Nobuhiro

机构信息

Department of Preventive Dentistry and Public Health, School of Dental Medicine, Tsurumi University,Japan.

出版信息

Eur J Dent. 2007 Jan;1(1):31-9.

OBJECTIVES

The evaluation of Mutans streptococci (MS) is one of the index for caries risk. Dentocult(TM) and CRT(TM) are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.

METHODS

The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.

RESULTS

The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium,CRT(TM), (P< .01) and Dentocult SM(TM) (P<.05).

CONCLUSION

The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor.

目的

变形链球菌（MS）的评估是龋病风险指标之一。Dentocult™和CRT™是用于便捷检测和评估MS的商业试剂盒。然而，MS的评估也可仅根据说明书进行。但该说明书在MS评估中使用并不方便。本研究的目的是检验改良的轻唾-杆菌肽（MSB）琼脂培养基与MSB琼脂培养基及商业试剂盒相比的实用性，并使用改良的MSB琼脂建立一种便捷的试剂盒（mMSB试剂盒）。

方法

采用MSB、改良MSB琼脂培养基和商业试剂盒检测27名受试者刺激唾液中的MS。对实验室和临床分离的MS菌株进行类似评估。通过酶联免疫吸附测定法比较检测到的细菌中MS的比例。

结果

列出了基于改良MSB琼脂培养基使用mMSB试剂盒得出的分数。唾液样本在培养方法和商业试剂盒之间显示出不同水平的MS。一些富含MS的样本未被商业试剂盒检测到。与MSB琼脂培养基、CRT™（P<0.01）和Dentocult SM™（P<0.05）相比，改良MSB琼脂培养基和mMSB试剂盒对MS的检测显著更高。