Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, China.
Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, China; Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ninghai 315604, China.
Comp Biochem Physiol B Biochem Mol Biol. 2024 Feb-Mar;270:110923. doi: 10.1016/j.cbpb.2023.110923. Epub 2023 Nov 11.
The blood clam (Tegillarca granosa), a marine bivalve of ecological and economic significance, often encounters intermittent hypoxia in mudflats and aquatic environments. To study the response of blood clam foot to prolonged intermittent hypoxia, the clams were exposed to intermittent hypoxia conditions (0.5 mg/L dissolved oxygen, with a 12-h interval) for 31 days. Initially, transcriptomic analysis was performed, uncovering a total of 698 differentially expressed genes (DEGs), with 236 upregulated and 462 downregulated. These genes show enrichments in signaling pathways related to glucose metabolism, sugar synthesis and responses to oxidative stress. Furthermore, the activity of the enzyme glutathione peroxidase (GPx) and the levels of gpx1 mRNA showed gradual increases, reaching their peak on the 13th day of intermittent hypoxia exposure. This observation suggests an indirect protective role of GPx against oxidative stress. The results of this study make a significantly contribute to our broader comprehensive of the physiological, biochemical responses, and molecular reactions governing the organization of foot muscle tissue in marine bivalves exposed to prolonged intermittent hypoxic conditions.
血蛤（Tegillarca granosa）是一种具有生态和经济意义的海洋双壳类动物，经常在泥滩和水生环境中遇到间歇性缺氧。为了研究血蛤足部对长时间间歇性缺氧的反应，将血蛤暴露于间歇性缺氧条件（0.5 mg/L 溶解氧，间隔 12 小时）下 31 天。最初进行了转录组分析，共发现了 698 个差异表达基因（DEGs），其中 236 个上调，462 个下调。这些基因在与葡萄糖代谢、糖合成和对氧化应激反应相关的信号通路中富集。此外，谷胱甘肽过氧化物酶（GPx）的活性和 gpx1 mRNA 的水平逐渐增加，在间歇性缺氧暴露的第 13 天达到峰值。这一观察表明 GPx 对氧化应激具有间接的保护作用。这项研究的结果为我们更全面地了解海洋双壳类动物在长时间间歇性缺氧条件下足部肌肉组织的生理、生化反应和分子反应做出了重要贡献。
