İspir Ünal, Mişe Yonar Serpil, Yonar M Enis, Silici Sibel
Malatya Turgut Özal University, Agriculture Faculty, Department of Fisheries, Malatya, Turkey.
Firat University, Fisheries Faculty, Department of Aquaculture, Elazig, Turkey.
Cell Mol Biol (Noisy-le-grand). 2023 Oct 31;69(10):179-188. doi: 10.14715/cmb/2023.69.10.26.
The present study was designed to assess the influence of dietary supplementation with chestnut bee pollen at various levels in rainbow trout, Oncorhynchus mykiss. For two weeks feeding period, a total of 300 fish were allocated into 12 fiberglass tanks and divided into four equal groups, three replicates each, with chestnut bee pollen (BP) dietary inclusion as follows; the fish group was given a basal diet (C); fish group fed a diet supplemented with BP 1% (BP-1); fish group fed a diet supplemented with BP 2% (BP-2); and fish group fed a diet supplemented with BP 4% (BP-3). At the end of the experiment, growth, haematological values, immune status, antioxidant status, and survival rate against Aeromonas salmonicida subsp. achromogenes were evaluated. Dietary supplementation with chestnut bee pollen significantly improves growth performance. Fish fed the diets containing chestnut bee pollen had higher the haematological values than those fed the control diet. The results showed that all the immunological parameters in the groups fed with chestnut bee pollen were significantly higher when compared to the control group. Moreover, dietary chestnut bee pollen increased disease resistance against Aeromonas salmonicida subsp. achromogenes compared to the control group. The tissue SOD, CAT and GSH-Px activities of groups fed with chestnut bee pollen significantly enhanced when compared with the control groups. In contrast, the tissue MDA levels in all groups fed with chestnut bee pollen were significantly decreased. The best values for the antioxidant parameters were determined in the groups fed with 2 and 4% of chestnut bee pollen. Overall, these findings suggest that dietary chestnut bee pollen enhances the growth, the haematological values, the immune and antioxidant response and increases disease resistance against rainbow trout.
本研究旨在评估在虹鳟（Oncorhynchus mykiss）日粮中添加不同水平板栗蜂花粉的影响。在为期两周的投喂期内，将总共300尾鱼分配到12个玻璃纤维水箱中，分为四个相等的组，每组三个重复，日粮中板栗蜂花粉（BP）的添加量如下：一组鱼投喂基础日粮（C）；一组鱼投喂添加1% BP的日粮（BP-1）；一组鱼投喂添加2% BP的日粮（BP-2）；一组鱼投喂添加4% BP的日粮（BP-3）。实验结束时，评估了生长、血液学值、免疫状态、抗氧化状态以及对杀鲑气单胞菌无色亚种的存活率。日粮中添加板栗蜂花粉显著提高了生长性能。投喂含板栗蜂花粉日粮的鱼的血液学值高于投喂对照日粮的鱼。结果表明，与对照组相比，投喂板栗蜂花粉组的所有免疫参数均显著更高。此外，与对照组相比，日粮中添加板栗蜂花粉提高了对杀鲑气单胞菌无色亚种的抗病能力。与对照组相比，投喂板栗蜂花粉组的组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性显著增强。相反，所有投喂板栗蜂花粉组的组织丙二醛（MDA）水平均显著降低。在投喂2%和4%板栗蜂花粉的组中，抗氧化参数的值最佳。总体而言，这些发现表明日粮中添加板栗蜂花粉可促进虹鳟的生长、提高血液学值、增强免疫和抗氧化反应并提高抗病能力。
