Lippert M C, Keefer D A
J Urol. 1987 Jan;137(1):140-5. doi: 10.1016/s0022-5347(17)43906-1.
The cellular distribution of androgen uptake was investigated in normal prostate and in Dunning R3327H prostate tumors in Copenhagen rats in vivo at different time intervals after castration. Quantitative dry-autoradiography was used to demonstrate which cell types have androgen binding, and to quantify the amount of androgen binding per cell type during initial castration-induced tumor regression and subsequent tumor relapse. Regardless of time after castration, tumor stromal nuclei had significantly more 3H-dihydrotestosterone (DHT) labelling than did tumor epithelial nuclei (p less than .001). On the other hand, prostate gland epithelial nuclei showed more 3H-DHT binding than prostate gland stromal nuclei. Tumor stromal nuclei had greater DHT uptake than any other cell type measured in the tumor or in secondary sex organs at all times after castration. Different cell types responded differently to castration. The 3H-DHT uptake measured in tumor stromal nuclei after castration showed that one day after castration 14.6 +/- 2.1 silver grains were present, 14 days after castration 9.3 +/- 2.4 were seen, and 50 days after castration 18.9 +/- 1.8 were present. This significant increase from 14 days to 50 days is not seen in the other cell types. This study gives insight into the cellular androgen receptor distribution in normal and malignant rat prostate tissue.
在去势后的不同时间间隔，对哥本哈根大鼠体内的正常前列腺组织和Dunning R3327H前列腺肿瘤组织中雄激素摄取的细胞分布进行了研究。采用定量干放射自显影技术来证明哪些细胞类型具有雄激素结合能力，并对去势诱导的肿瘤初始消退及随后复发过程中每种细胞类型的雄激素结合量进行定量分析。无论去势后的时间如何，肿瘤基质细胞核的3H-双氢睾酮（DHT）标记均显著多于肿瘤上皮细胞核（p<0.001）。另一方面，前列腺腺上皮细胞核的3H-DHT结合量多于前列腺腺基质细胞核。在去势后的所有时间点，肿瘤基质细胞核的DHT摄取量均高于在肿瘤或第二性器官中检测的任何其他细胞类型。不同细胞类型对去势的反应不同。去势后在肿瘤基质细胞核中测得的3H-DHT摄取量显示，去势后1天有14.6±2.1个银粒，去势后14天有9.3±2.4个银粒，去势后50天有18.9±1.8个银粒。从14天到50天的这种显著增加在其他细胞类型中未见到。本研究深入了解了正常和恶性大鼠前列腺组织中的细胞雄激素受体分布情况。
