Gastineau Romain, Dąbek Przemysław, Mianowicz Kamila, Stoyanova Valcana, Krawcewicz Artur, Abramowski Tomasz
Institute of Marine and Environmental Sciences, University of Szczecin, ul. Mickiewicza 16a, Szczecin, 70-383, Poland University of Szczecin Szczecin Poland.
Interoceanmetal Joint Organization, ul. Cyryla i Metodego 9-9A, Szczecin, 71-541, Poland Interoceanmetal Joint Organization Szczecin Poland.
Zookeys. 2023 Oct 31;1183:81-98. doi: 10.3897/zookeys.1183.109000. eCollection 2023.
The Clarion-Clipperton Zone (CCZ) in the tropical East Pacific is a region of interest for deep-sea mining due to its underwater deposits of polymetallic nodules containing economically important metals such as nickel, copper, and cobalt. It is also a region of extensive baseline studies aiming to describe the state of the environment, including the biodiversity of the benthic fauna. An abundant component of the abyssal plain ecosystem consists of sessile fauna which encrusts polymetallic nodules and are vulnerable to potential impacts arising from exploitation activities, particularly removal of substrate. Therefore, this fauna is often considered to have key species whose genetic connectivity should be studied to assess their ecological resilience. One such species is Cairns, 2015, a deep-sea coral from the CCZ whose presence in the Interoceanmetal Joint Organization (IOM) claim area has been confirmed during samplings. In this study, we used next-generation sequencing (NGS) to obtain the nuclear rRNA gene and the complete mitochondrial genome of from IOM exploration area. The mitogenome is 18,825 bp long and encodes for 14 protein coding genes, 2 rRNAs, and a single tRNA. The two phylogeny reconstructions derived from these data confirm previous studies and display within a highly supported cluster of seven species whose mitogenomes are all colinear and of comparable size. This study also demonstrates the suitability of NGS for DNA barcoding of the benthic megafauna of the CCZ, which could become part of the IOM protocol for the assessment of population diversity and genetic connectivity in its claim area.
热带东太平洋的克拉里昂-克利珀顿区（CCZ）因其富含镍、铜和钴等具有经济重要性金属的多金属结核水下矿床，而成为深海采矿的关注区域。它也是一个进行广泛基线研究的区域，旨在描述环境状况，包括底栖动物的生物多样性。深海平原生态系统的一个丰富组成部分是固着动物群，它们附着在多金属结核上，容易受到开采活动产生的潜在影响，特别是底物的移除。因此，这种动物群通常被认为有一些关键物种，其遗传连通性应该被研究以评估它们的生态恢复力。其中一个这样的物种是2015年发现的凯恩斯深海珊瑚，它来自CCZ，在国际海洋金属联合组织（IOM）的申请区域采样期间已被证实存在。在本研究中，我们使用下一代测序（NGS）来获取来自IOM勘探区域该物种的核rRNA基因和完整线粒体基因组。线粒体基因组长度为18,825 bp，编码14个蛋白质编码基因、2个rRNA和一个tRNA。从这些数据得出的两种系统发育重建证实了先前的研究，并显示该物种处于一个高度支持的七个物种的聚类中，其线粒体基因组都是共线的且大小相当。本研究还证明了NGS适用于CCZ底栖大型动物的DNA条形码分析，这可能成为IOM在其申请区域评估种群多样性和遗传连通性协议的一部分。
