Tokita H, Tanaka N, Ueno T, Fujimoto S, Nakano K
Gan No Rinsho. 1986 Nov;32(14):1803-8.
We have developed a simple in vitro chemosensitivity test for breast cancer. Tumor tissues were chopped finely with razor blade. The cell clumps were pounced into tissue culture medium, which contained a specified level of concentration of anticancer drugs, and incubated at 37 degrees C for four to eight hours. Nine to 10 kinds of drugs were tested on each specimen. After incubation, the clumps were pumped down. The cells were smeared and stained with Giemsa for microscopic examination. The individual tumors showed different sensitivities towards various drugs, and the typical morphological changes observed in their nuclei; were karyorrhexis and karyopyknosis.
我们已经开发出一种针对乳腺癌的简单体外化学敏感性测试方法。用剃须刀片将肿瘤组织切碎。将细胞团块接种到含有特定浓度抗癌药物的组织培养基中，在37℃下孵育4至8小时。对每个标本测试9至10种药物。孵育后，将细胞团块沉淀下来。将细胞涂片并用吉姆萨染色以进行显微镜检查。各个肿瘤对各种药物表现出不同的敏感性，并且在其细胞核中观察到的典型形态学变化为核碎裂和核固缩。
