Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives, Kunming 650032, China.
Bioresour Technol. 2022 Jan;344(Pt A):126214. doi: 10.1016/j.biortech.2021.126214. Epub 2021 Oct 29.
A major challenge facing by astaxanthin industrialization is the low productivity and high production costs. This study established a two-stage cultivation strategy based on the application of NaCl to improve the production of biomass and astaxanthin by Haematococcus pluvialis. During the first growth stage, 12.5 mg L NaCl led to a remarkable enhancement in biomass, which was 1.28 times compared with the control. Moreover, 2 g L NaCl stimulated the astaxanthin content from 12.18 mg g to 25.92 mg g during the second induction stage. Simultaneously, salinity stress application increased the lipids and GABA contents, as well as the levels of Ca and carotenogenic genes' expression, but suppressed the contents of carbohydrate and protein and high-light induced-ROS. This study proposed a simple and convenient strategy for efficient coproduction of biomass and astaxanthin and provides insights into the underlying mechanism of astaxanthin biosynthesis in H. pluvialis induced by salinity stress.
虾青素产业化面临的主要挑战是生产力低和生产成本高。本研究建立了基于 NaCl 应用的两段式培养策略，以提高雨生红球藻的生物质和虾青素的生产。在第一生长阶段，12.5mg/L 的 NaCl 导致生物质显著增加，与对照组相比增加了 1.28 倍。此外，在第二诱导阶段，2g/L 的 NaCl 将虾青素含量从 12.18mg/g 刺激到 25.92mg/g。同时，盐度胁迫的应用增加了脂质和 GABA 的含量，以及 Ca 和类胡萝卜素生物合成基因表达水平，但抑制了碳水化合物和蛋白质的含量以及高光诱导的 ROS。本研究提出了一种高效生产生物质和虾青素的简单方便的策略，并深入了解了盐度胁迫诱导雨生红球藻虾青素生物合成的潜在机制。
