Pokrovskiĭ S N, Adamova I Iu, Babiĭ A V
Kardiologiia. 1986 Oct;26(10):49-54.
The authors introduce a new approach in a clinical and experimental research termed immunosorption. They have developed highly specific and selective agarose-conjugated immunosorbent with monospecific goat antibodies against human LDL. The sorbent is used in clinical practice for removal of atherogenic lipoproteins from the patients plasma--LDL apheresis procedure. The advantages of affinity chromatography based on LDL-apheresis over plasma-pheresis and haemosorption are discussed. Nonspecific adsorptive, hydrophobic and electrostatic interactions that take place during haemosorption do not provide the required selectivity, while immunosorption procedure enables minimization of nonspecific adsorption of plasma compounds during LDL-apheresis. Several examples illustrate clinical applications of immunosorption method.
作者们在一项名为免疫吸附的临床和实验研究中引入了一种新方法。他们研发出了一种高度特异性和选择性的琼脂糖偶联免疫吸附剂，其带有针对人类低密度脂蛋白（LDL）的单特异性山羊抗体。这种吸附剂用于临床实践，从患者血浆中去除致动脉粥样硬化脂蛋白——低密度脂蛋白去除术。文中讨论了基于低密度脂蛋白去除术的亲和色谱法相对于血浆去除术和血液吸附法的优势。血液吸附过程中发生的非特异性吸附、疏水和静电相互作用无法提供所需的选择性，而免疫吸附程序能够在低密度脂蛋白去除术期间将血浆化合物的非特异性吸附降至最低。几个例子说明了免疫吸附法的临床应用。
