College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
South China Center of National Saline-tolerant Rice Technology Innovation Center, Zhanjiang, 524088, China.
BMC Plant Biol. 2023 Nov 14;23(1):558. doi: 10.1186/s12870-023-04595-z.
Salt stress severely restricts rapeseed growth and productivity. Hemin can effectively alleviate salt stress in plants. However, the regulatory effect of Hemin on rapeseed in salt stress is unclear. Here, we analyzed the response and remediation mechanism of Hemin application to rapeseed before and after 0.6% (m salt: m soil) NaCl stress. Experiment using two Brassica napus (AACC, 2n = 38) rapeseed varieties Huayouza 158R (moderately salt-tolerant) and Huayouza 62 (strongly salt-tolerant). To explore the best optional ways to improve salt stress resistance in rapeseed.
Our findings revealed that exogenous application of Hemin enhanced morph-physiological traits of rapeseed and significantly attenuate the inhibition of NaCl stress. Compared to Hemin (SH) treatment, Hemin (HS) significantly improved seedlings root length, seedlings height, stem diameter and accumulated more dry matter biomass under NaCl stress. Moreover, Hemin (HS) significantly improved photosynthetic efficiency, activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and decreased electrolyte leakage (EL) and malondialdehyde (MDA) content, thus resulting in the alleviation of oxidative membrane damage. Hemin (HS) showed better performance than Hemin (SH) under NaCl stress.
Hemin could effectively mitigate the adverse impacts of salt stress by regulating the morph-physiological, photosynthetic and antioxidants traits of rapeseed. This study may provide a basis for Hemin to regulate cultivated rapeseed salt tolerance and explore a better way to alleviate salt stress.
盐胁迫严重限制油菜生长和生产力。血红素可以有效缓解植物的盐胁迫。然而,血红素对油菜在盐胁迫下的调节作用尚不清楚。在这里,我们分析了油菜在 0.6%(盐:土)NaCl 胁迫前后应用血红素的响应和修复机制。实验采用两种甘蓝型油菜(AACC,2n=38)油菜品种华优杂 158R(中度耐盐)和华优杂 62(耐盐性强)。探讨提高油菜耐盐性的最佳可选途径。
研究结果表明,外源性血红素的应用增强了油菜的形态生理特性,并显著减轻了 NaCl 胁迫的抑制作用。与血红素(SH)处理相比,血红素(HS)在 NaCl 胁迫下显著提高了幼苗根长、幼苗高度、茎直径和积累更多的干物质生物量。此外,血红素(HS)显著提高了光合效率,超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)等抗氧化酶的活性,降低了电解质泄漏(EL)和丙二醛(MDA)含量,从而减轻了氧化膜的损伤。血红素(HS)在 NaCl 胁迫下比血红素(SH)表现更好。
血红素通过调节油菜的形态生理、光合和抗氧化特性,能有效减轻盐胁迫的不利影响。本研究可为血红素调控栽培油菜耐盐性提供依据,并探索一种更好的缓解盐胁迫的方法。