Binhai Agriculture College, Guangdong Ocean University, Zhanjiang, 524088, China.
South China Center of National Saline-tolerant Rice Technology Innovation Center, Zhanjiang, 524088, China.
BMC Plant Biol. 2024 Sep 30;24(1):904. doi: 10.1186/s12870-024-05586-4.
The growth and yield of rapeseed are significantly hampered by salt stress. Indole-3-butyric Acid Potassium Salt (IBA-K) has been found to alleviate the impact of salt stress on plant growth. However, the regulatory effect of IBA-K dipping on salt-stressed rapeseed remains unclear. To explore the implications of IBA-K on the growth and development of rapeseed during the seedling stage, we conducted potting experiments using the Huayouza 62 variety. Five different concentrations of IBA-K for seed soaking (0, 10, 20, 40, 80 mg·L) were tested. The promotional impact of IBA-K on rapeseed demonstrated an initial increase followed by a decline, reaching a peak at 20 mg·L. Therefore, 20 mg·L was determined as the optimal concentration for subsequent experiments. To further understand the mechanism of IBA-K's action on salt-stressed rapeseed seedlings, we utilized the moderately salt-resistant cabbage rapeseed variety Huayouza 158R and the highly salt-resistant Huayouza 62 as specimens. The investigation focused on their response and repair mechanisms under 150 mmol·L NaCl stress. The findings demonstrated that compared with the sole NaCl stress, the 20 mg·L IBA-K seed soaking treatment under salt stress significantly enhanced the plant height, stem diameter, and leaf area of both rapeseed varieties. It also led to greater biomass accumulation, increased chlorophyll content, and improved photosynthetic efficiency in rapeseed. Furthermore, this treatment bolstered the activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), while significantly reducing the levels of electrolyte leakage (EL) and malondialdehyde (MDA). Consequently, it alleviated the membrane lipid peroxidation damage induced by NaCl stress, enhanced the accumulation of soluble proteins, maintained cellular osmotic pressure, and effectively mitigated the adverse effects of NaCl stress on rapeseed.
盐胁迫显著抑制油菜的生长和产量。吲哚丁酸钾盐（IBA-K）已被发现可减轻盐胁迫对植物生长的影响。然而，IBA-K 浸种对盐胁迫油菜的调控作用尚不清楚。为了探讨 IBA-K 对油菜幼苗生长发育的影响，我们进行了盆栽试验，选用华优杂 62 号品种。试验测试了 5 种不同浓度的 IBA-K 浸种（0、10、20、40、80mg·L）。IBA-K 对油菜的促进作用表现为先增加后减少，在 20mg·L 时达到峰值。因此，20mg·L 确定为后续实验的最佳浓度。为了进一步了解 IBA-K 对盐胁迫油菜幼苗作用的机制，我们选用耐盐性中等的白菜型油菜华优杂 158R 和耐盐性强的华优杂 62 号为材料。研究聚焦于它们在 150mmol·L NaCl 胁迫下的响应和修复机制。结果表明，与单独 NaCl 胁迫相比，盐胁迫下 20mg·L IBA-K 浸种处理显著提高了两种油菜品种的株高、茎粗和叶面积。它还导致油菜生物量积累增加、叶绿素含量增加和光合作用效率提高。此外，这种处理增强了超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等抗氧化酶的活性，同时显著降低了电解质渗漏（EL）和丙二醛（MDA）的水平。因此，它缓解了 NaCl 胁迫引起的膜脂过氧化损伤，增加了可溶性蛋白的积累，维持了细胞渗透压，有效减轻了 NaCl 胁迫对油菜的不利影响。
