HSCCC 结合在线 DPPH-HPLC 分析从 Maxim. 中靶向分离和纯化抗氧化剂。
Target-Guided Isolation and Purification of Antioxidants from Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis.
机构信息
School of Life Science, Qinghai Normal University, Xining 810008, China.
Agricultural and Rural Science and Technology Guidance Development Service Center of Qinghai Province, Xining 810008, China.
出版信息
Molecules. 2023 Oct 29;28(21):7332. doi: 10.3390/molecules28217332.
Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from using HSCCC guided by online DPPH-HPLC analysis. Firstly, the online DPPH-HPLC analysis was performed to profile out the antioxidant active molecules in The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the targeted antioxidant active molecules could be well enriched under the optimized extraction conditions. Then, the antioxidant active molecules were separated by high-speed countercurrent chromatography ethyl acetate/-butanol/water (2:3:5, //) as the solvent system. Finally, the three targets including 16.8 mg of Isovitexin, 9.8 mg of Isoorientin, and 26.7 mg of Apigenin-6,8-di-C-β-d-glucopyranoside were obtained from 100 mg of sample. Their structures were identified by H NMR spectroscopy.
麻叶荨麻在中医药中被广泛应用，因其具有很强的抗氧化性能。本研究采用在线 DPPH-HPLC 分析指导的高速逆流色谱法从麻叶荨麻中分离纯化了 3 种抗氧化剂。首先，通过在线 DPPH-HPLC 分析对麻叶荨麻中的抗氧化活性分子进行了分析。采用响应面法优化了超声辅助提取条件，结果表明，在优化的提取条件下，目标抗氧化活性分子可以得到很好的富集。然后，采用乙酸乙酯/-丁醇/水（2:3:5， //）作为溶剂系统，通过高速逆流色谱法对抗氧化活性分子进行分离。最后，从 100mg 样品中得到了 3 个目标化合物，分别为 16.8mg 的异荭草苷、9.8mg 的异牡荆苷和 26.7mg 的芹菜素-6,8-二-C-β-D-吡喃葡萄糖苷。通过 1H NMR 光谱对它们的结构进行了鉴定。
Zotero 插件