Rojas-Muñoz Yesica Vanesa, Santagapita Patricio Román, Quintanilla-Carvajal María Ximena
Maestría en Diseño y Gestión de Procesos, Facultad de Ingeniería, Campus Universitario del Puente del Común, Universidad de La Sabana, Chía 250001, Colombia.
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires & Centro de Investigación en Hidratos de Carbono (CIHIDECAR, UBA-CONICET), Buenos Aires 1428, Argentina.
Polymers (Basel). 2023 Nov 1;15(21):4296. doi: 10.3390/polym15214296.
The stability and release properties of all bioactive capsules are strongly related to the composition of the wall material. This study aimed to evaluate the effect of the wall materials during the encapsulation process by ionotropic gelation on the viability of K73, a lactic acid bacterium that has hypocholesterolemia probiotic potential. A response surface methodology experimental design was performed to improve bacterial survival during the synthesis process and under simulated gastrointestinal conditions by tuning the wall material composition (gelatin 25% /, sweet whey 8% /, and sodium alginate 1.5% /). An optimal mixture formulation determined that the optimal mixture must contain a volume ratio of 0.39/0.61 / sweet whey and sodium alginate, respectively, without gelatin, with a final bacterial concentration of 9.20 log CFU/mL. The mean particle diameter was 1.6 ± 0.2 mm, and the experimental encapsulation yield was 95 ± 3%. The INFOGEST model was used to evaluate the survival of probiotic beads in gastrointestinal tract conditions. Upon exposure to in the vitro conditions of oral, gastric, and intestinal phases, the encapsulated cells of decreased only by 0.32, 0.48, and 1.53 log CFU/mL, respectively, by employing the optimized formulation, thereby improving the survival of probiotic bacteria during both the encapsulation process and under gastrointestinal conditions compared to free cells. Beads were characterized using SEM and ATR-FTIR techniques.
所有生物活性胶囊的稳定性和释放特性与壁材组成密切相关。本研究旨在评估离子凝胶法包封过程中壁材对具有降胆固醇益生菌潜力的乳酸菌K73活力的影响。通过调整壁材组成（明胶25% /、甜乳清8% /和海藻酸钠1.5% /），采用响应面法实验设计来提高合成过程中和模拟胃肠道条件下细菌的存活率。确定的最佳混合物配方为，最佳混合物必须分别包含体积比为0.39/0.61的甜乳清和海藻酸钠，不含明胶，最终细菌浓度为9.20 log CFU/mL。平均粒径为1.6±0.2 mm，实验包封率为95±3%。INFOGEST模型用于评估益生菌珠在胃肠道条件下的存活率。采用优化配方后，在口腔、胃和肠道阶段的体外条件下，包封细胞分别仅减少0.32、0.48和1.53 log CFU/mL，从而与游离细胞相比，提高了益生菌在包封过程中和胃肠道条件下的存活率。使用扫描电子显微镜（SEM）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）技术对珠子进行了表征。
