封装于棕榈油纳米脂质载体中的枣椰提取物对预期抗菌效果的影响

Date Palm Extract () Encapsulated into Palm Oil Nanolipid Carrier for Prospective Antibacterial Influence.

作者信息

Elsewedy Heba S, Shehata Tamer M, Alqahtani Nashi K, Khalil Hany Ezzat, Soliman Wafaa E

机构信息

Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Alhofuf 31982, Al-Ahsa, Saudi Arabia.

Department of Pharmaceutical Sciences, College of Pharmacy, Almaarefa University, Dariyah, Riyadh 13713, Saudi Arabia.

出版信息

Plants (Basel). 2023 Oct 25;12(21):3670. doi: 10.3390/plants12213670.

DOI:10.3390/plants12213670

PMID:37960029

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10648499/

Abstract

It is worthwhile to note that using natural products today has shown to be an effective strategy for attaining the therapeutic goal with the highest impact and the fewest drawbacks. In Saudi Arabia, date palm (Phoenix dactylifera) is considered the principal fruit owing to its abundance and incredible nutritional benefits in fighting various diseases. The main objective of the study is to exploit the natural products as well as the nanotechnology approach to obtain great benefits in managing disorders. The present investigation focused on using the powder form of date palm extract (DPE) of Khalas cultivar and incorporates it into a nanolipid formulation such as a nanostructured lipid carrier (NLC) prepared with palm oil. Using the quality by design (QbD) methodology, the most optimized formula was chosen based on the number of assigned parameters. For more appropriate topical application, the optimized DP-NLC was combined with a pre-formulated hydrogel base forming the DP-NLC-hydrogel. The developed DP-NLC-hydrogel was evaluated for various physical properties including pH, viscosity, spreadability, and extrudability. Additionally, the in vitro release of the formulation as well as its stability upon storage under two different conditions of room temperature and refrigerator were investigated. Eventually, different bacterial strains were utilized to test the antibacterial efficacy of the developed formulation. The optimized DP-NLC showed proper particle size (266.9 nm) and in vitro release 77.9%. The prepared DP-NLC-hydrogel showed acceptable physical properties for topical formulation, mainly, pH 6.05, viscosity 9410 cP, spreadability 57.6 mm, extrudability 84.5 (g/cm), and in vitro release 42.4%. Following three months storage under two distinct conditions, the formula exhibited good stability. Finally, the antibacterial activity of the developed DP-NLC-hydrogel was evaluated and proved to be efficient against various bacterial strains.

摘要

值得注意的是，如今使用天然产物已被证明是实现治疗目标的一种有效策略，其影响最大且缺点最少。在沙特阿拉伯，椰枣（Phoenix dactylifera）因其产量丰富以及在对抗各种疾病方面具有令人难以置信的营养益处而被视为主要水果。该研究的主要目的是利用天然产物以及纳米技术方法，在疾病管理方面获得巨大益处。本研究专注于使用哈拉斯品种椰枣提取物（DPE）的粉末形式，并将其纳入纳米脂质制剂，如用棕榈油制备的纳米结构脂质载体（NLC）。采用质量源于设计（QbD）方法，根据指定参数的数量选择了最优化的配方。为了更适合局部应用，将优化后的DP-NLC与预先配制的水凝胶基质混合，形成DP-NLC-水凝胶。对所制备的DP-NLC-水凝胶进行了各种物理性质的评估，包括pH值、粘度、铺展性和可挤出性。此外，还研究了该制剂的体外释放情况以及在室温与冰箱两种不同条件下储存时的稳定性。最后，使用不同的细菌菌株来测试所制备制剂的抗菌效果。优化后的DP-NLC显示出合适的粒径（266.9 nm）和77.9%的体外释放率。所制备的DP-NLC-水凝胶显示出适用于局部制剂的物理性质，主要为pH值6.05、粘度9410 cP、铺展性57.6 mm、可挤出性84.5（g/cm）以及42.4%的体外释放率。在两种不同条件下储存三个月后，该配方表现出良好的稳定性。最后，对所制备的DP-NLC-水凝胶的抗菌活性进行了评估，结果证明其对各种细菌菌株均有效。