Wizenberg Sydney B, Muir-Guarnaccia Jillian, Campbell Lesley G
Department of Chemistry and Biology, Toronto Metropolitan University, 350 Victoria St, Toronto, ON M5B 2K3, Canada.
Department of Biology, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
Plants (Basel). 2023 Oct 31;12(21):3731. doi: 10.3390/plants12213731.
L. is cultivated globally for its cannabinoid-dense inflorescences. Commercial preference for sinsemilla has led to the development of methods for producing feminized seeds through cross-pollination of cosexual (masculinized) female plants. Although the induction of cosexuality in plants is common, to date, no work has empirically tested how masculinization of female plants impacts male flowering, pollen production, pollen fitness, and related life-history trade-offs. Here, we cultivated a population of plants (CFX-2) and explored how the route to cosexuality (drought vs. chemical induction) impacted flowering phenology, pollen production, and pollen fitness, relative to unsexual male plants. Unisexual males flowered earlier and longer than cosexual plants and produced 223% more total pollen (F = 74.41, < 0.001), but per-flower pollen production did not differ across reproductive phenotypes (F = 0.887, = 0.427). Pollen viability was 200% higher in unisexual males and drought-induced cosexuals (F = 189.70, < 0.001). Pollen non-abortion rates only differed in a marginally significant way across reproductive phenotypes (F = 3.00, = 0.06). Here, we demonstrate that masculinization of female plants impacts whole-plant pollen production and pollen fitness in .
L.因其富含大麻素的花序而在全球范围内种植。商业上对无籽大麻的偏好促使人们开发出通过两性（雄性化）雌株异花授粉来生产雌性化种子的方法。尽管在植物中诱导两性现象很常见，但迄今为止，尚无研究实证检验雌株雄性化如何影响雄花开花、花粉产生、花粉适应性以及相关的生活史权衡。在此，我们培育了一批植物（CFX - 2），并探究相对于单性雄株，两性现象的诱导途径（干旱诱导与化学诱导）如何影响开花物候、花粉产生和花粉适应性。单性雄株比两性植株开花更早且花期更长，总花粉产量高出223%（F = 74.41，P < 0.001），但每朵花的花粉产量在不同生殖表型之间没有差异（F = 0.887，P = 0.427）。单性雄株和干旱诱导的两性植株的花粉活力高出200%（F = 189.70，P < 0.001）。花粉不流产率在不同生殖表型之间仅存在微弱的显著差异（F = 3.00，P = 0.06）。在此，我们证明雌株雄性化会影响L.整株植物的花粉产量和花粉适应性。
