Saimi Gulisitan, Wang Ziyu, Liusui Yunhao, Guo Yanjun, Huang Gengqing, Zhao Huixin, Zhang Jingbo
Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi 830054, China.
Plants (Basel). 2023 Nov 2;12(21):3755. doi: 10.3390/plants12213755.
Drought stress imposes severe constraints on crop growth and yield. The NAC transcription factors (TF) play a pivotal role in regulating plant stress responses. However, the biological functions and regulatory mechanisms of many cotton NACs have not been explored. In this study, we report the cloning and characterization of , a gene encoding a typical cotton NAC TF. The expression of was induced by PEG treatment, drought stress, and ABA treatment. Furthermore, we investigated its function using the virus-induced gene silencing (VIGS) method. silenced plants exhibited a poorer growth status under drought stress conditions compared to the controls. The silenced cotton plants had a lower leaf relative water and chlorophyll content and a higher MDA content compared to the controls under the drought treatment. The levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) enzyme activity in the silenced plants were found to be lower compared to the controls when exposed to drought stress. Additionally, the downregulation of the drought stress-related genes, , , , , , , , , and , was observed in the silenced cotton. Together, our research reveals that plays a role in the reaction of cotton to drought stress by affecting the expression of genes related to drought stress. The data obtained from this study lay the theoretical foundation for further in-depth research on the biological function and regulatory mechanisms of .
干旱胁迫对作物生长和产量造成严重限制。NAC转录因子在调节植物应激反应中起关键作用。然而，许多棉花NAC的生物学功能和调控机制尚未得到探索。在本研究中，我们报告了一个编码典型棉花NAC转录因子的基因的克隆和特征。该基因的表达受聚乙二醇（PEG）处理、干旱胁迫和脱落酸（ABA）处理诱导。此外，我们使用病毒诱导基因沉默（VIGS）方法研究了其功能。与对照相比，该基因沉默的植株在干旱胁迫条件下生长状况较差。在干旱处理下，该基因沉默的棉花植株与对照相比，叶片相对含水量和叶绿素含量较低，丙二醛（MDA）含量较高。在干旱胁迫下，该基因沉默的植株中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的酶活性水平低于对照。此外，在该基因沉默的棉花中观察到干旱胁迫相关基因的下调。总之，我们的研究表明该基因通过影响干旱胁迫相关基因的表达在棉花对干旱胁迫的反应中发挥作用。本研究获得的数据为进一步深入研究该基因的生物学功能和调控机制奠定了理论基础。
