Hu Ziyao, Lei Jianfeng, Dai Peihong, Liu Chao, Wugalihan Abuduweili, Liu Xiaodong, Li Yue
College of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, China.
College of Agronomy, Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, China.
Plants (Basel). 2022 Jun 15;11(12):1580. doi: 10.3390/plants11121580.
As a plant-specific Rho-like small G protein, the ROP (Rho-related GTPase of plants) protein regulates the growth and development of plants and various stress responses in the form of molecular switches. Drought is a major abiotic stress that limits cotton yield and fiber quality. In this study, virus-induced gene silencing (VIGS) technology was used to analyze the biological function of in cotton drought stress tolerance. Meanwhile, we used yeast two-hybrid and bimolecular fluorescence complementation assays to examine the interaction between GhROP3 and GhGGB. has a high expression level in cotton true leaves and roots, and responds to drought, high salt, cold, heat stress, and exogenous abscisic acid (ABA) and auxin (IAA) treatments. Silencing improved the drought tolerance of cotton. The water loss rates (WLR) of detached leaves significantly reduced in silenced plants. Also, the relative water content (RWC) and total contents of chlorophyll (Chl) and proline (Pro) of leaves after drought stress and the activities of three antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) significantly increased, whereas the contents of hydrogen peroxide (HO) and malondialdehyde (MDA) significantly reduced. In the leaves of silenced plants, the expression of genes related to ABA synthesis and its related pathway was significantly upregulated, and the expression of decomposition-related gene and genes related to IAA synthesis and its related pathways was significantly downregulated. It indicated that was a negative regulator of cotton response to drought by participating in the negative regulation of the ABA signaling pathway and the positive regulation of the IAA signaling pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhROP3 protein interacted with the GhGGB protein in vivo and in vitro. This study provided a theoretical basis for the in-depth investigation of the drought resistance-related molecular mechanism of the gene and the biological function of the gene.
作为一种植物特有的类Rho小G蛋白，ROP（植物Rho相关GTP酶）蛋白以分子开关的形式调节植物的生长发育和各种应激反应。干旱是限制棉花产量和纤维品质的主要非生物胁迫。在本研究中，利用病毒诱导基因沉默（VIGS）技术分析了[基因名称未给出]在棉花干旱胁迫耐受性中的生物学功能。同时，我们使用酵母双杂交和双分子荧光互补试验来检测GhROP3和GhGGB之间的相互作用。[基因名称未给出]在棉花真叶和根中具有高表达水平，并对干旱、高盐、低温、热胁迫以及外源脱落酸（ABA）和生长素（IAA）处理有响应。沉默[基因名称未给出]提高了棉花的耐旱性。沉默植株中离体叶片的失水率（WLR）显著降低。此外，干旱胁迫后叶片的相对含水量（RWC）、叶绿素（Chl）和脯氨酸（Pro）的总含量以及三种抗氧化酶过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性显著增加，而过氧化氢（HO）和丙二醛（MDA）的含量显著降低。在沉默植株的叶片中，与ABA合成及其相关途径相关的基因表达显著上调，与分解相关的[基因名称未给出]基因以及与IAA合成及其相关途径相关的基因表达显著下调。这表明[基因名称未给出]通过参与ABA信号通路的负调控和IAA信号通路的正调控，是棉花对干旱响应的负调节因子。酵母双杂交和双分子荧光互补试验表明，GhROP3蛋白在体内和体外均与GhGGB蛋白相互作用。本研究为深入探究[基因名称未给出]基因的抗旱相关分子机制及[基因名称未给出]基因的生物学功能提供了理论依据。
