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NAC转录因子MdNAC29对苹果的耐旱性起负调控作用。

The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple.

作者信息

Li Sen, Jing Xiuli, Tan Qiuping, Wen Binbin, Fu Xiling, Li Dongmei, Chen Xiude, Xiao Wei, Li Ling

机构信息

College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China.

State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.

出版信息

Front Plant Sci. 2023 Jul 6;14:1173107. doi: 10.3389/fpls.2023.1173107. eCollection 2023.

DOI:10.3389/fpls.2023.1173107
PMID:37484477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10359905/
Abstract

Drought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple gene and investigated its role in regulating drought tolerance. Subcellular localization experiments showed that was localized to the nucleus and transcription was induced by the PEG treatment. Over-expression of reduced drought tolerance in apple plants, calli, and tobacco, and exhibited higher relative conductivity, malondialdehyde (MDA) content, and lower chlorophyll content under drought stress. The transcriptomic analyses revealed that reduced drought resistance by modulating the expression of photosynthesis and leaf senescence-related genes. The qRT-PCR results showed that overexpression of repressed the expression of drought-resistance genes. Yeast one-hybrid and dual-luciferase assays demonstrated that MdNAC29 directly repressed expression. Moreover, the yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that MdNAC29 interacted with the MdPP2-B10 (F-box protein), which responded to drought stress, and MdPP2-B10 enhanced the repressive effect of MdNAC29 on the transcriptional activity of the . Taken together, our results indicate that is a negative regulator of drought resistance, and provide a theoretical basis for further molecular mechanism research.

摘要

干旱胁迫是一种影响全球农业生产的不利刺激因素。NAC转录因子参与植物的发育和生长，同时在非生物胁迫响应中发挥不同作用。在此，我们分离了苹果基因并研究其在调节耐旱性中的作用。亚细胞定位实验表明，该基因定位于细胞核，且转录受聚乙二醇（PEG）处理诱导。该基因的过表达降低了苹果植株、愈伤组织和烟草的耐旱性，在干旱胁迫下表现出更高的相对电导率、丙二醛（MDA）含量和更低的叶绿素含量。转录组分析表明，该基因通过调节光合作用和叶片衰老相关基因的表达来降低抗旱性。qRT-PCR结果显示，该基因的过表达抑制了抗旱基因的表达。酵母单杂交和双荧光素酶实验表明，MdNAC29直接抑制该基因的表达。此外，酵母双杂交和双分子荧光互补实验表明，MdNAC29与响应干旱胁迫的MdPP2-B10（F-box蛋白）相互作用，且MdPP2-B10增强了MdNAC29对该基因转录活性的抑制作用。综上所述，我们的结果表明该基因是抗旱性的负调控因子，为进一步研究分子机制提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/cb74f9ac0cf2/fpls-14-1173107-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/93699add18c6/fpls-14-1173107-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/1c6eaf6c8dbc/fpls-14-1173107-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/a0b02c85bc36/fpls-14-1173107-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/e9c363fa4593/fpls-14-1173107-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/e0596c32ff3f/fpls-14-1173107-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/24fb39b1b2f1/fpls-14-1173107-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/5518e9d40e02/fpls-14-1173107-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/cb74f9ac0cf2/fpls-14-1173107-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/93699add18c6/fpls-14-1173107-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/1c6eaf6c8dbc/fpls-14-1173107-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/a0b02c85bc36/fpls-14-1173107-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/e9c363fa4593/fpls-14-1173107-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/e0596c32ff3f/fpls-14-1173107-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/24fb39b1b2f1/fpls-14-1173107-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/5518e9d40e02/fpls-14-1173107-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c00/10359905/cb74f9ac0cf2/fpls-14-1173107-g008.jpg

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