维生素D3联合PhytoSolve对多囊卵巢综合征NMRI小鼠模型着床相关基因的影响：一项实验研究
The Effect of Vitamin D3 Supplemented with PhytoSolve on Genes Involved in Implantation in an NMRI Mice Model of Polycystic Ovary Syndrome: An Experimental Study.
作者信息
Hakimpour Sahar, Govahi Azam, Eghbali Sahar, Shabani Ronak, Mehdizadeh Rana, Ajdary Marziyeh, Mehdizadeh Mehdi
机构信息
Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.
出版信息
JBRA Assist Reprod. 2023 Nov 14;27(4):694-701. doi: 10.5935/1518-0557.20230064.
OBJECTIVE
Vitamin D3 has been shown to be effective in the treatment of PCOS. However, due to its poor solvability and bioavailability, effective time is delayed and dosage requirements are increased. In our previous study, we demonstrated that PhytoSolve containing VD3 is more effective than vitamin D3 alone in the treatment of PCOS. In this study, we aimed to investigate the effect of this vitamin D3 formulation on gene expression involved in implantation in patients with PCOS.
METHODS
To create PhytoSolve, Lipid S75, glycerol, and MCT oil were combined using a sonicator probe. Six groups, each consisting of 36 female Naval Medical Research Institute (NMRI) mice, were included in the following groups: control; sham; PCOS; PhytoSolve; PhytoSolve containing VD3; and vitamin D3. The mice were given DHEA injections to induce PCOS. After administering PhytoSolve containing VD3 and vitamin D3 by gavage for one week from the 13th day of model creation, the female mice were mated and endometrial tissue was collected for analysis of LIF, β-integrin, and HOXA10 proteins and genes.
RESULTS
Compared to the group receiving vitamin D3 alone, the group receiving PhytoSolve containing vitamin D3 showed a significant increase in the expression of LIF, β-integrin, and HOXA10 genes (p<0.05). Although there was an increase in the expression of β-integrin and HOXA10 proteins in the group given PhytoSolve containing vitamin D3 compared to the group given vitamin D3, this increase was not significant. However, the increase in LIF protein expression in the group given PhytoSolve containing vitamin D3 was significant when compared to the group given vitamin D3 (p<0.05).
CONCLUSIONS
The use of PhytoSolve containing vitamin D3 was more effective than vitamin D3 alone. The PhytoSolve formulation might be a useful solution for medications with limited solubility and bioavailability.
目的
已证明维生素D3对多囊卵巢综合征（PCOS）的治疗有效。然而，由于其溶解性和生物利用度差，起效时间延迟且剂量需求增加。在我们之前的研究中，我们证明含维生素D3的植物溶解剂（PhytoSolve）在治疗PCOS方面比单独使用维生素D3更有效。在本研究中，我们旨在研究这种维生素D3制剂对PCOS患者着床相关基因表达的影响。
方法
为制备植物溶解剂，使用超声探头将脂质S75、甘油和中链甘油三酯（MCT）油混合。以下分组包括六组，每组由36只雌性海军医学研究所（NMRI）小鼠组成：对照组；假手术组；PCOS组；植物溶解剂组；含维生素D3的植物溶解剂组；维生素D3组。给小鼠注射脱氢表雄酮（DHEA）以诱导PCOS。从模型建立的第13天起，通过灌胃给予含维生素D3的植物溶解剂和维生素D3一周后，将雌性小鼠进行交配，并收集子宫内膜组织用于分析白血病抑制因子（LIF）、β整合素和同源框A10（HOXA10）蛋白及基因。
结果
与单独接受维生素D3的组相比，接受含维生素D3的植物溶解剂的组中LIF、β整合素和HOXA10基因的表达显著增加（p<0.05）。虽然与给予维生素D3的组相比，给予含维生素D3的植物溶解剂的组中β整合素和HOXA10蛋白的表达有所增加，但这种增加不显著。然而，与给予维生素D3的组相比，给予含维生素D3的植物溶解剂的组中LIF蛋白表达的增加是显著的（p<0.05）。
结论
使用含维生素D3的植物溶解剂比单独使用维生素D3更有效。植物溶解剂制剂可能是解决溶解性和生物利用度有限的药物的有用方法。
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