滋养层条件培养基和滋养层衍生的细胞外囊泡抑制人中耳上皮细胞中的脂多糖诱导的炎症反应。

Inhibition of lipopolysaccharide-induced inflammation by trophoblast-conditioned medium and trophoblast-derived extracellular vesicles in human middle ear epithelial cells.

机构信息

Division of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea.

Center for Health Care Convergence at Korea University Guro Hospital, Seoul, Republic of Korea.

出版信息

Sci Rep. 2023 Nov 14;13(1):19822. doi: 10.1038/s41598-023-46731-7.

DOI:10.1038/s41598-023-46731-7

PMID:37963902

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10645728/

Abstract

Otitis media is a common disease but can cause severe inner ear inflammation and hearing loss if it persists for more than two weeks. This study elucidates the inflammation-inhibiting efficacy of conditioned medium (CM) and extracellular vesicles (EVs) derived from human trophoblast (TB) cells in lipopolysaccharide (LPS)-induced human middle ear epithelial cells (HMEECs). TB-conditioned medium (TB-CM) reduced the inflammatory response and regulated mucin and epithelial sodium channel genes in LPS-induced HMEECs. The underlying mechanism of cell migration during inflammatory healing in LPS-induced HMEECs treated with TB-CM was determined by RNA-sequencing analysis. Specifically, the NF-κB pathway related to the copper metabolism MURR1 domain protein was studied and verified through siRNA. This elucidation of the anti-inflammatory effect of TB-CM and TB-derived EVs demonstrates their clinical potential to treat chronic inflammation.

摘要

中耳炎是一种常见疾病，但如果持续超过两周，可能会导致严重的内耳炎症和听力损失。本研究阐明了人滋养层细胞（TB）来源的条件培养基（CM）和细胞外囊泡（EV）在脂多糖（LPS）诱导的人中耳上皮细胞（HMEEC）中的抗炎作用。TB 条件培养基（TB-CM）降低了 LPS 诱导的 HMEEC 中的炎症反应，并调节了粘蛋白和上皮钠通道基因。通过 RNA 测序分析，确定了 LPS 诱导的 HMEEC 在用 TB-CM 处理后的炎症愈合过程中的细胞迁移的潜在机制。具体而言，研究了与铜代谢 MURR1 结构域蛋白相关的 NF-κB 通路，并通过 siRNA 进行了验证。这项关于 TB-CM 和 TB 衍生 EV 的抗炎作用的阐明表明它们具有治疗慢性炎症的临床潜力。