lncRNA MALAT-1在新型冠状病毒肺炎感染中的表达模式及其通过miR-200c-3p和SIRT1对疾病严重程度的潜在影响

Expression patterns of lncRNA MALAT-1 in SARS-COV-2 infection and its potential effect on disease severity via miR-200c-3p and SIRT1.

作者信息

Shaker Olfat, El Amir Monica, Elfatah Yasmine Abd, Elwi Heba M

机构信息

Medical Biochemistry and Molecular Biology, Kasr Alainy Faculty of Medicine, Cairo University, Kasralainy st, Cairo, 11562, Egypt.

Internal Medicine, Kasr Alainy Faculty of Medicine, Cairo University, Kasralainy st, Cairo, 11562, Egypt.

出版信息

Biochem Biophys Rep. 2023 Nov 1;36:101562. doi: 10.1016/j.bbrep.2023.101562. eCollection 2023 Dec.

UNLABELLED

Downregulating Angiotensin Converting Enzyme2 (ACE2) expression may be a shared mechanism for RNA viruses.

AIM

Evaluate the expressions of ACE2 effectors: the long non-coding RNA 'MALAT-1', the micro-RNA 'miR-200c-3p' and the histone deacetylase 'SIRT1' in SARS-COV-2 patients and correlate to disease severity. Sera samples from 98 SARS-COV-2 patients and 30 healthy control participants were collected. qRT-PCR was used for MALAT-1 and miR-200c-3p expression. SIRT1 was measured using ELISA.

RESULTS

In sera of COVID-19 patients, gene expression of miR-200c-3p is increased while MALAT-1 is decreased. SIRT1 protein level is decreased (P value < 0.001). Findings are accentuated with increased disease severity. Serum MALAT-1, miR-200c-3p and SIRT1 could be used as diagnostic markers at cut off values of 0.04 (95.9 % sensitivity), 5.59 (94.9 % sensitivity, 99 % specificity), and 7.4 (98 % sensitivity) respectively. A novel MALAT-1-miR-200c-3p-SIRT1 pathway may be involved in the regulation of SARS-COV-2 severity.

未标记

下调血管紧张素转换酶2（ACE2）表达可能是RNA病毒的一种共同机制。

目的

评估严重急性呼吸综合征冠状病毒2（SARS-CoV-2）患者中ACE2效应物的表达：长链非编码RNA“MALAT-1”、微小RNA“miR-200c-3p”和组蛋白脱乙酰酶“SIRT1”，并将其与疾病严重程度相关联。收集了98例SARS-CoV-2患者和30名健康对照参与者的血清样本。采用qRT-PCR检测MALAT-1和miR-200c-3p的表达。使用酶联免疫吸附测定法（ELISA）检测SIRT1。

结果

在2019冠状病毒病（COVID-19）患者的血清中，miR-200c-3p的基因表达增加，而MALAT-1减少。SIRT1蛋白水平降低（P值<0.001）。随着疾病严重程度的增加，这些发现更加明显。血清MALAT-1、miR-200c-3p和SIRT1分别在截断值为0.04（灵敏度95.9%）、5.59（灵敏度94.9%，特异性99%）和7.4（灵敏度98%）时可作为诊断标志物。一条新的MALAT-1-miR-200c-3p-SIRT1途径可能参与SARS-CoV-2严重程度的调节。