Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, 341000, China.
National Engineering Research Center for Modernization of Traditional Chinese Medicine-Hakka Medical Resources Branch, College of Pharmacy, Gannan Medical University, Ganzhou, 341000, China.
Chem Biodivers. 2023 Dec;20(12):e202301449. doi: 10.1002/cbdv.202301449. Epub 2023 Nov 29.
One new fawcettimine-type alkaloid, phlecarinadine A (1), and twelve known ones (2-13) were isolated from the whole plant of Phlegmariurus carinatus. Their chemical structures were unambiguously established by extensive spectroscopic analyses, including nuclear magnetic resonance (NMR) spectroscopic and high resolution electrospray ionization mass spectrometry (HR-ESI-MS). The absolute configuration of 1 was elucidated by the electronic circular dichroism (ECD) technique. These compounds were tested for their cytotoxic and neuroprotective activities. None of these compounds revealed cytotoxic activity against five tumor cells. Phlegmariurine B (2) exhibited potential neuroprotective effects against hemin-induced HT22 cell damage, with a 17.76 % increase in cell survival at 10 μM. In further study, 2 can ameliorate hemin-induced neuronal cell death via an anti-apoptotic pathway. These findings suggest that 2 might be a valuable lead compound with neuroprotective activity.
从 Phlegmariurus carinatus 的全株植物中分离得到一种新的法卡林胺型生物碱，即 phlecarinadine A（1），以及 12 种已知化合物（2-13）。通过广泛的光谱分析，包括核磁共振（NMR）光谱和高分辨电喷雾电离质谱（HR-ESI-MS），明确确定了它们的化学结构。通过电子圆二色性（ECD）技术阐明了 1 的绝对构型。对这些化合物进行了细胞毒性和神经保护活性测试。这些化合物对五种肿瘤细胞均无细胞毒性。Phlegmariurine B（2）对血红素诱导的 HT22 细胞损伤表现出潜在的神经保护作用，在 10 μM 时细胞存活率增加了 17.76%。进一步的研究表明，2 可以通过抗凋亡途径改善血红素诱导的神经元细胞死亡。这些发现表明，2 可能是一种具有神经保护活性的有价值的先导化合物。
