Department of Gynecology and Obstetrics, Tenth People's Hospital, Tongji University, Shanghai, PR China.
Institute of Gynecological Minimal Invasive Medicine, Tongji University School of Medicine, Shanghai, PR China.
Gynecol Endocrinol. 2023 Dec;39(1):2269265. doi: 10.1080/09513590.2023.2269265. Epub 2023 Nov 15.
To investigate the expression of HMGB1 and toll-like receptor 4 (TLR4) in adenomyosis eutopic/ectopic endometrium.
Twenty patients with adenomyosis and 20 controls, all undergoing laparoscopy, were recruited from September 2015 to July 2016. Samples were collected from the endometrium without adenomyosis (CE), the eutopic endometrium with adenomyosis (EuE), and the ectopic endometrium with adenomyosis (EE). The mRNA and protein expression of HMGB1 and TLR4, and interleukin-6 (IL-6) and interleukin-8 (IL-8) RNA expression levels were measured.
The average age of the adenomyosis women was 43.4 ± 5.3 years; their BMI was 23.3 ± 2.3 kg/m. The control group included women aged 38.8 ± 9.8 years, with BMI 22.2 ± 3.4 kg/m. The mRNA expression levels of HMGB1, TLR4, IL-6, and IL-8 in the EE and EuE groups were higher than those in the CE group ( < .01), and those in the EE group were higher than those in the EuE group ( < .01). The protein expression levels of HMGB1 and TLR4 in the EE and EuE groups were higher than those in the CE group ( < .01); they were higher in the EE group than the ones in the EuE group ( < .01). HMGB1 mRNA was significantly positively correlated with TLR4 in EuE and EC patients ( = 0.538 and = 0.916, < .01), as well as with IL-6 ( = 0.470 and = 0.976, < .01) and IL-8 ( = 0.574 and = 0.650, < .01).
The overexpression of HMGB1 and TLR4 in EuE and EE is positively correlated with IL-6 and IL-8 expression. The HMGB1 signaling-mediated immune-inflammatory system might be involved in the development of adenomyosis.
研究高迁移率族蛋白 B1（HMGB1）和 Toll 样受体 4（TLR4）在子宫腺肌病在位/异位子宫内膜中的表达。
2015 年 9 月至 2016 年 7 月，通过腹腔镜收集 20 例子宫腺肌病患者和 20 例对照者的子宫内膜标本，无子宫腺肌病的子宫内膜（CE）、在位子宫内膜伴子宫腺肌病（EuE）和异位子宫内膜伴子宫腺肌病（EE）。检测 HMGB1、TLR4 及白细胞介素-6（IL-6）、白细胞介素-8（IL-8）mRNA 表达水平。
子宫腺肌病患者的平均年龄为 43.4±5.3 岁，BMI 为 23.3±2.3kg/m；对照组年龄为 38.8±9.8 岁，BMI 为 22.2±3.4kg/m。EE 和 EuE 组中 HMGB1、TLR4、IL-6 和 IL-8 的 mRNA 表达水平均高于 CE 组（ < .01），EE 组高于 EuE 组（ < .01）；HMGB1 和 TLR4 蛋白表达水平在 EE 和 EuE 组中均高于 CE 组（ < .01），EE 组高于 EuE 组（ < .01）。EuE 和 EC 患者中 HMGB1 mRNA 与 TLR4 显著正相关（ = 0.538 和 = 0.916， < .01），与 IL-6（ = 0.470 和 = 0.976， < .01）和 IL-8（ = 0.574 和 = 0.650， < .01）也显著正相关。
EuE 和 EE 中 HMGB1 和 TLR4 的过度表达与 IL-6 和 IL-8 的表达呈正相关。HMGB1 信号转导介导的免疫炎症系统可能参与了子宫腺肌病的发生。
