Department of Plant Breeding, Physiology and Seed Science, University of Agriculture, Krakow, Poland.
Sci Rep. 2023 Nov 15;13(1):19931. doi: 10.1038/s41598-023-47318-y.
Due to global warming, winter hardiness may seem to become less important for plant survival and yield. However, this is a superficial assumption, as probably only the most important factors locally affecting plant overwintering will change. For example, the frequency, degree, and length of extreme winter warming events may increase, leading to de-acclimation of plants. This study aimed to investigate existing variability in de-acclimation tolerance in Polish winter barley breeding materials and European winter and facultative barley cultivars, and to identify accessions with the highest and the lowest tolerance to de-acclimation by means of visual estimation of regrowth after freezing, measurements of electrolyte leakage and chlorophyll fluorescence, and LT50 assessment. The results of this study showed that freezing tolerance and tolerance to de-acclimation are independent traits, and even highly freezing tolerant plants can be susceptible to de-acclimation. Our results highlight the role of photosynthetic apparatus in de-acclimation, proving that chlorophyll fluorescence parameters, especially ET/CS, can be useful indicators of tolerance to de-acclimation. This study also confirmed that although the mechanisms of response to de-acclimation seem to be common for susceptible barley accessions, the mechanisms of tolerance are different, and may be related to the accession's origin.
由于全球变暖，冬季抗寒性似乎对植物的生存和产量变得不那么重要。然而，这只是一种表面的假设，因为可能只有当地影响植物越冬的最重要因素会发生变化。例如，极端冬季变暖事件的频率、程度和持续时间可能会增加，导致植物去驯化。本研究旨在调查波兰冬季大麦育成材料和欧洲冬季兼性大麦品种中去驯化耐受性的现有变异性，并通过冷冻后生长的目视估计、电解质渗漏和叶绿素荧光测量以及 LT50 评估来确定对去驯化具有最高和最低耐受性的品系。本研究结果表明，抗冻性和去驯化耐受性是独立的性状，即使是高度抗冻的植物也可能容易去驯化。我们的研究结果强调了光合作用器在去驯化中的作用，证明叶绿素荧光参数，特别是 ET/CS，可以作为去驯化耐受性的有用指标。本研究还证实，尽管对敏感大麦品系的去驯化反应机制似乎是共同的，但耐受性的机制是不同的，可能与品系的起源有关。
