Li Hongliang, Yu Hongjin, Su Wentao, Wang Haitao, Tan Mingqian
State Key Lab of Marine Food Processing & Safety Control, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China.
National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
ACS Omega. 2023 Oct 27;8(44):41537-41547. doi: 10.1021/acsomega.3c05542. eCollection 2023 Nov 7.
Multicore alginate microspheres (MCPs) have been demonstrated as promising carriers for bioactive substances. Herein, the influence of the size of the inner core on the bioaccessibility of astaxanthin (AST) was investigated using both in vitro and in vivo methods. MCPs with different inner core sizes were fabricated in which the oil-in-water emulsion with different oil droplet sizes was embedded in alginate microspheres (AST@MCPs) via the electrospray technology. The AST@MCPs appeared as a uniform sphere with an average size of 300 μm. The AST encapsulation efficiency in the AST@MCPs was determined to be more than 68%, which was independent of the inner core size. The bioaccessibility of AST increased from 38.3 to 83.2% as the size of the inner core decreased. Furthermore, the anti-inflammatory activity of AST@MCPs after in vitro simulated digestion was evaluated by LPS-induced RAW264.7 cells. The results suggested that AST@MCPs with a smaller inner core size exhibited a stronger anti-inflammatory activity, which further proved the results obtained from in vitro simulated digestion. As expected, the oral administration of AST@MCPs significantly mitigated colitis symptoms in DSS-induced ulcerative colitis mice. Compared with AST@MCPs with larger inner cores, AST@MCPs with smaller inner cores reflect stronger anti-inflammatory activity in vivo. These results suggested that the bioaccessibility of AST in MCPs increased significantly with the decrease in the inner core size, which may be attributed to the rapid formation of micelles in the intestine. This work provides a simple and efficient strategy to prepare microspheres for the enhanced delivery of AST, which has important implications for the design of health-promoting foods.
多核藻酸盐微球（MCPs）已被证明是生物活性物质的有前途的载体。在此，使用体外和体内方法研究了内核大小对虾青素（AST）生物可及性的影响。制备了具有不同内核大小的MCPs，其中通过电喷雾技术将具有不同油滴大小的水包油乳液包埋在藻酸盐微球（AST@MCPs）中。AST@MCPs呈均匀球体，平均大小为300μm。AST@MCPs中AST的包封率被确定超过68%，这与内核大小无关。随着内核尺寸的减小，AST的生物可及性从38.3%提高到83.2%。此外，通过脂多糖诱导的RAW264.7细胞评估了体外模拟消化后AST@MCPs的抗炎活性。结果表明，内核尺寸较小的AST@MCPs表现出更强的抗炎活性，这进一步证明了体外模拟消化获得的结果。正如预期的那样，口服AST@MCPs显著减轻了右旋糖酐硫酸钠诱导的溃疡性结肠炎小鼠的结肠炎症状。与内核较大的AST@MCPs相比，内核较小的AST@MCPs在体内表现出更强的抗炎活性。这些结果表明，MCPs中AST的生物可及性随着内核尺寸的减小而显著增加，这可能归因于肠道中胶束的快速形成。这项工作为制备用于增强AST递送的微球提供了一种简单有效的策略，这对促进健康食品的设计具有重要意义。
