Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Anal Chem. 2023 Nov 28;95(47):17347-17353. doi: 10.1021/acs.analchem.3c03695. Epub 2023 Nov 16.
Selective recognition of short oligonucleotides at the single-molecule level is particularly important for early disease detection and treatment. In this work, polydopamine (PDA)-coated nanopores were prepared via self-polymerization as a solid-state nanopore sensing platform for the recognition of oligonucleotide C (PolyC). The PDA coating possesses abundant active sites, such as indole, amino, carboxyl, catechol, and quinone structures, which had interactions with short oligonucleotides to slow down the translocation rate. PDA-coated nanopores selectively interact with PolyC by virtue of differences in hydrogen bonding forces, generating a larger blocking current, while polyA and polyT demonstrated very small blockings. At the same time, PDA-coated nanopores can sensitively distinguish PolyC with different lengths, such as 20, 14, and 10 nt. The functionalization of PDA on the solid-state nanopore provides an opportunity for the rational design of the recognition surface for biomolecules.
在单分子水平上对短寡核苷酸进行选择性识别对于早期疾病检测和治疗尤为重要。在这项工作中，通过自聚合制备了聚多巴胺 (PDA) 涂层纳米孔作为固体纳米孔传感平台，用于识别寡核苷酸 C（PolyC）。PDA 涂层具有丰富的活性位点，如吲哚、氨基、羧基、儿茶酚和醌结构，它们与短寡核苷酸相互作用以降低迁移率。PDA 涂层纳米孔通过氢键力的差异选择性地与 PolyC 相互作用，产生更大的阻断电流，而 polyA 和 polyT 则表现出非常小的阻断。同时，PDA 涂层纳米孔可以灵敏地区分不同长度的 PolyC，例如 20、14 和 10 nt。PDA 在固体纳米孔上的功能化为生物分子识别表面的合理设计提供了机会。
