School of Control and Computer Engineering, North China Electric Power University, Beijing, 102206, China.
School of Electronics Engineering and Computer Science, Peking University, Beijing, 100871, China.
Small. 2023 Nov;19(47):e2303715. doi: 10.1002/smll.202303715. Epub 2023 Jul 26.
The detection of biomolecules at the single molecule level has important applications in the fields of biosensing and biomedical diagnosis. The solid-state nanopore (SS nanopore) is a sensitive tool for detecting single molecules because of its unique label-free and low sample consumption properties. SS nanopore translocation of small biomolecules is typically driven by an electronic field force and is thus influenced by the charge, shape, and size of the target molecules. Therefore, it remains challenging to control the translocation of biomolecules through SS nanopores, particularly for different proteins with complex conformations and unique charges. Toward this problem, a DNA polyhedral carrier coating strategy to assist protein translocation through SS nanopores is developed, which facilitates target protein detection. The current signal-to-noise ratios are improved significantly using this DNA carrier loading strategy. The proposed method should aid the detection of proteins, which are difficult to translocate through nanopores. This coating-assisted method offers a wide range of applications for SS nanopore detection and promotes the development of single-molecule detection.
在生物传感和生物医学诊断领域，在单分子水平上检测生物分子具有重要的应用。固态纳米孔（SS 纳米孔）是一种用于检测单分子的敏感工具，因为它具有独特的无标记和低样品消耗的特性。小分子通过 SS 纳米孔的易位通常由电场力驱动，因此受到目标分子的电荷、形状和大小的影响。因此，控制生物分子通过 SS 纳米孔的易位仍然具有挑战性，特别是对于具有复杂构象和独特电荷的不同蛋白质。针对这一问题，开发了一种 DNA 多面体载体涂层策略来辅助 SS 纳米孔中的蛋白质易位，从而便于目标蛋白质的检测。使用这种 DNA 载体加载策略，当前的信噪比得到了显著提高。该方法应该有助于检测难以通过纳米孔易位的蛋白质。这种涂层辅助方法为 SS 纳米孔检测提供了广泛的应用，并促进了单分子检测的发展。
