Altern Ther Health Med. 2024 Jul;30(7):214-221.
Acute radiodermatitis is a type of skin injury caused by tumor radiotherapy. Compound Dahuang Baiji spray (CDBS) is a traditional Chinese medicine spray made from Dahuang and Baiji decoction. In the present study, we aimed to investigate the effects and mechanisms of CDBS on radiation dermatitis. We analyzed the main components of CDBS using High Performance Liquid Chromatography (HPLC). Through network pharmacology prediction, the target of Dahuang and Baiji was identified as arachidonate 5-lipoxygenase (ALOX5), associated with inflammation. Therefore, we constructed radiodermatitis rat models and treated them with CDBS for 14 d. Skin samples were collected from the rats' injured skin tissues, and pathological changes, oxidative stress indicators, inflammatory cytokines, and ALOX5 expression were detected using techniques such as HE staining, blood parameters analysis, ELISA, Real-time qPCR, and Western blot. The characteristic appearances of radiodermatitis were observed in different rat groups which indicated that the skin injury score in the model group was at grade II and was at grade I in the CDBS group. In addition, the HE results showed that CDBS reduced the necrosis of collagen fibers and inflammatory cell infiltration in the dermis of the radiodermatitis rats. Moreover, compared to the model group, CDBS significantly decreased leukocytes, lymphocytes, and neutrophils in the blood, as well as levels of IL-2, LTB4, 5-LO, NO, and ALOX5 expression in rat blood. Our findings suggest the therapeutic effect of CDBS on radiodermatitis by downregulating ALOX5 to inhibit inflammation, potentially serving as a radiodermatitis therapy.
急性放射性皮炎是一种由肿瘤放疗引起的皮肤损伤。复方大黄白芨喷雾剂（CDBS）是一种由大黄和白芨汤制成的中药喷雾剂。在本研究中，我们旨在研究 CDBS 对放射性皮炎的作用和机制。我们使用高效液相色谱法（HPLC）分析了 CDBS 的主要成分。通过网络药理学预测，大黄和白芨的靶点被鉴定为花生四烯酸 5-脂氧合酶（ALOX5），与炎症有关。因此，我们构建了放射性皮炎大鼠模型，并使用 CDBS 治疗 14 天。从大鼠受伤皮肤组织中采集皮肤样本，通过 HE 染色、血液参数分析、ELISA、实时 qPCR 和 Western blot 等技术检测病理变化、氧化应激指标、炎症细胞因子和 ALOX5 表达。不同大鼠组观察到放射性皮炎的特征表现，表明模型组的皮肤损伤评分达到 II 级，而 CDBS 组达到 I 级。此外，HE 结果表明，CDBS 减少了放射性皮炎大鼠真皮中胶原纤维的坏死和炎症细胞浸润。与模型组相比，CDBS 还显著降低了血液中的白细胞、淋巴细胞和中性粒细胞，以及大鼠血液中 IL-2、LTB4、5-LO、NO 和 ALOX5 表达水平。我们的研究结果表明，CDBS 通过下调 ALOX5 抑制炎症来治疗放射性皮炎，可能成为一种放射性皮炎的治疗方法。
