Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Zhejiang Provincial Key Lab of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Hangzhou, Zhejiang, China.
Invest Ophthalmol Vis Sci. 2023 Nov 1;64(14):20. doi: 10.1167/iovs.64.14.20.
To observe the similarities and differences between herpes simplex virus type 1 (HSV-1) and murine cytomegalovirus (MCMV)-induced viral anterior uveitis (VAU), both in vitro and in vivo.
Primary rat trabecular meshwork cells (RTMCs) were infected by HSV-1 or MCMV to clarify the pattern of virus replication and the effect on cells. In vivo, intracameral injection of HSV-1 or MCMV was performed to establish the VAU rat models. The clinical manifestation, intraocular pressure (IOP), histological characteristics, ultrastructural changes, and the expression of inflammatory cytokines in the anterior segment were observed and compared between these two types of VAU models.
Both viruses could infect the RTMCs but HSV-1 exhibited an earlier and greater cytopathic effect in vitro. In vivo, both VAU rats showed typical acute VAU signs, and the IOP elevation seemed to be correlated with the inflammatory progression. Histopathological findings and ultrastructural changes revealed tissue damage and cell infiltration in the anterior chamber angle. In both models, similar proinflammatory cytokines were upregulated. HSV-1 and MCMV viral particles were identified under transmission electron microscopy.
HSV-1 and MCMV infection share certain similarities but have significant differences both in vitro and in vivo. HSV-1 usually has a stronger anterior segment inflammation with a longer duration compared with MCMV in VAU models. Our results provided a valuable animal model for investigating pathogenesis and exploring therapeutic strategies for clinical VAU.
观察单纯疱疹病毒 1 型（HSV-1）和鼠巨细胞病毒（MCMV）诱导的病毒性前葡萄膜炎（VAU）在体外和体内的相似性和差异性。
原代大鼠小梁网细胞（RTMC）分别感染 HSV-1 或 MCMV，以阐明病毒复制模式及其对细胞的影响。体内采用玻璃体内注射 HSV-1 或 MCMV 建立 VAU 大鼠模型。观察并比较这两种 VAU 模型的临床症状、眼内压（IOP）、组织学特征、超微结构变化以及眼前段炎症细胞因子的表达。
两种病毒均可感染 RTMC，但 HSV-1 在体外具有更早和更大的细胞病变效应。体内，两种 VAU 大鼠均表现出典型的急性 VAU 体征，IOP 升高似乎与炎症进展相关。组织病理学和超微结构观察发现眼前房角有组织损伤和细胞浸润。两种模型中均上调了相似的促炎细胞因子。电镜下可观察到 HSV-1 和 MCMV 病毒颗粒。
HSV-1 和 MCMV 感染在体外和体内具有某些相似性，但也存在显著差异。与 MCMV 相比，HSV-1 通常在前葡萄膜炎模型中具有更强的眼前段炎症，持续时间更长。我们的研究结果为研究发病机制和探索临床 VAU 的治疗策略提供了有价值的动物模型。
