Liu Yi, Cao Yueping
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Plant Mol Biol. 2023 Nov;113(4-5):157-170. doi: 10.1007/s11103-023-01380-2. Epub 2023 Nov 16.
Drought affects soybean growth and ultimately led to yield reduction. WRKY transcription factors involve in the regulation of abiotic stress. Few functions of WRKY transcription factors underlying drought tolerance in soybean are clear. Here, we reported a WRKY transcription factor named GmWRKY17 that positively regulates soybean drought tolerance by regulating drought-induced genes and ABA-related genes. Transcriptome sequencing (RNA-Seq) and yeast one hybrid analysis identified downstream genes regulated by GmWRKY17. ChIP-qPCR, EMSA and dual-luciferase reporter assay showed that GmWRKY17 directly bound to the promoters of the GmDREB1D and GmABA2, and activated their expression under drought stress. Overexpression of GmDREB1D gene enhanced drought tolerance of soybean. Taken together, our study revealed a regulatory mechanism that GmWRKY17 transcription factor may improve soybean drought tolerance by mediating ABA synthesis and DREB signaling pathway.
干旱影响大豆生长并最终导致产量降低。WRKY转录因子参与非生物胁迫的调控。大豆中WRKY转录因子在耐旱性方面的功能鲜为人知。在此，我们报道了一个名为GmWRKY17的WRKY转录因子，它通过调控干旱诱导基因和ABA相关基因来正向调节大豆的耐旱性。转录组测序（RNA-Seq）和酵母单杂交分析确定了受GmWRKY17调控的下游基因。ChIP-qPCR、EMSA和双荧光素酶报告基因检测表明，GmWRKY17直接结合到GmDREB1D和GmABA2的启动子上，并在干旱胁迫下激活它们的表达。GmDREB1D基因的过表达增强了大豆的耐旱性。综上所述，我们的研究揭示了一种调控机制，即GmWRKY17转录因子可能通过介导ABA合成和DREB信号通路来提高大豆的耐旱性。
