Northeast Agricultural University, Harbin 150001, China.
Int J Mol Sci. 2024 Jun 26;25(13):6972. doi: 10.3390/ijms25136972.
Drought stress is one of the significant abiotic stresses that limit soybean ( [L.] Merr.) growth and production. Ankyrin repeat (ANK) proteins, being highly conserved, occupy a pivotal role in diverse biological processes. genes were classified into nine subfamilies according to conserved domains in the soybean genome. However, the function of ANK-TM subfamily proteins (Ankyrin repeat proteins with a transmembrane domain) in the abiotic-stress response to soybean remains poorly understood. In this study, we first demonstrated the subcellular localization of GmANKTM21 in the cell membrane and nucleus. Drought stress-induced mRNA levels of , which encodes proteins belonging to the ANK-TM subfamily, Transgenic soybean improved drought tolerance at the germination and seedling stages, with higher stomatal closure in soybean, lower water loss, lower malondialdehyde (MDA) content, and less reactive oxygen species (ROS) production compared with the wild-type soybean (Dongnong50). RNA-sequencing (RNA-seq) and RT-qPCR analysis of differentially expressed transcripts in overexpression of further identified potential downstream genes, including , , and , which showed higher expression in transgenic soybean, than those in wild-type soybean and KEGG enrichment analysis showed that MAPK signaling pathways were mostly enriched in overexpressing soybean plants under drought stress conditions. Therefore, we demonstrate that plays an important role in tolerance to drought stress in soybeans.
干旱胁迫是限制大豆（[L.] Merr.）生长和生产的主要非生物胁迫之一。锚蛋白重复（ANK）蛋白高度保守，在多种生物过程中起着关键作用。根据大豆基因组中的保守结构域，将 基因分为九个亚家族。然而，ANK-TM 亚家族蛋白（具有跨膜结构域的锚蛋白重复蛋白）在大豆非生物胁迫响应中的功能仍知之甚少。在本研究中，我们首先证明了 GmANKTM21 在细胞膜和核中的亚细胞定位。干旱胁迫诱导编码 ANK-TM 亚家族蛋白的 的 mRNA 水平上调，与野生型大豆相比，转 大豆在萌发和幼苗期提高了耐旱性，表现为大豆气孔关闭更多，水分损失更少，丙二醛（MDA）含量更低，活性氧（ROS）生成更少。与野生型大豆（Dongnong50）相比，差异表达转录物的 RNA 测序（RNA-seq）和 RT-qPCR 分析进一步鉴定了潜在的下游基因，包括 、 、和 ，它们在转基因大豆中的表达高于野生型大豆，KEGG 富集分析表明，在干旱胁迫条件下，MAPK 信号通路在 过表达的大豆植株中富集最多。因此，我们证明 在大豆耐受干旱胁迫中发挥重要作用。
