Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia City, Minia, Egypt.
Department of Botany and Microbiology, Faculty of Science, Minia University, Minia, 61519, Egypt.
BMC Microbiol. 2023 Nov 16;23(1):343. doi: 10.1186/s12866-023-03085-4.
Endophytic fungi, particularly from higher plants have proven to be a rich source of antimicrobial secondary metabolites. The purpose of this study is to examine the antimicrobial potential of three endophytic fungi Aspergillus sp. SA1, Aspergillus sp. SA2, and Aspergillus sp. SA3, cultivated from Nigella sativa seeds against Staphylococcus aureus (ATCC 9144), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 13883), MRSA (ATCC 33591), and human pathogen Candida albicans (ATCC 10231). Furthermore, the most active cultivated endophytic fungi were molecularly identified via internal transcribed spacer (ITS) sequencing. HR-ESIMS guided approach has been used successfully in chemical profiling of 26 known bioactive secondary metabolites (1-26), which belongs to different classes of natural compounds such as polyketides, benzenoids, quinones, alcohols, phenols or alkaloids. Finally, in-silico interactions within active site of fungal Cyp51 and bacterial DNA gyrase revealed possibility of being a hit-target for such metabolites as antimicrobials.
内生真菌，特别是高等植物内生真菌已被证明是抗菌次生代谢产物的丰富来源。本研究旨在研究从黑种草种子中分离得到的三种内生真菌（Aspergillus sp. SA1、Aspergillus sp. SA2 和 Aspergillus sp. SA3）的抗菌潜力，以对抗金黄色葡萄球菌（ATCC 9144）、大肠杆菌（ATCC 25922）、铜绿假单胞菌（ATCC 27853）、肺炎克雷伯菌（ATCC 13883）、耐甲氧西林金黄色葡萄球菌（MRSA，ATCC 33591）和人类病原体白色念珠菌（ATCC 10231）。此外，通过内部转录间隔区（ITS）测序对最具活性的培养内生真菌进行了分子鉴定。高分辨电喷雾串联质谱（HR-ESIMS）引导的方法已成功用于 26 种已知生物活性次生代谢产物（1-26）的化学分析，这些次生代谢产物属于不同类别的天然化合物，如聚酮、苯丙素、醌类、醇类、酚类或生物碱。最后，真菌 Cyp51 和细菌 DNA 拓扑异构酶活性位点内的相互作用表明，这些代谢产物可能作为抗菌药物的潜在作用靶点。
