Laboratory of Experimental Clinical Gastroenterology (LIM-07), Division of Clinical Gastroenterology and Hepatology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil.
Lipids Laboratory (LIM-10), Division of Endocrinology and Metabolism, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil.
Clinics (Sao Paulo). 2023 Nov 15;78:100305. doi: 10.1016/j.clinsp.2023.100305. eCollection 2023.
Treatments of Inflammatory Bowel Disease (IBD) are able to control symptoms in most cases, however, a fraction of patients do not improve or have a loss of response to treatments, making it important to explore new therapeutic strategies. Hyperbaric oxygen therapy (HBO) may represent one of them. The aim of this study was to evaluate the effects of HBO therapy in an experimental model of IBD.
Sixty male BALBc mice were divided into six groups. Group 1 was colitis-induced with trinitrobenzene sulfonic acid (TNBS) + ethanol, group 2 received TNBS + ethanol plus HBO, group 3 received only ethanol, group 4 received ethanol plus HBO, group 5 received saline solution, and group 6 received saline solution plus HBO. HBO was performed for four days, subsequently, the mice were evaluated daily. At the end of the study, samples from the intestine were collected for histological analysis as well as for measurement of antioxidant enzymes and cytokine levels.
HBO significantly improved the clinical and histological status of the animals. Treatment with HBO increased the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in all of the groups; moreover, the difference was only significant between the TNBS and TNBS + HBO groups and treatments promoted a reduction in the proinflammatory cytokines IFN-γ, IL-12, IL-17 and TNF-α and increased the anti-inflammatory cytokines IL-4 and IL-10, with no changes in IL-13.
HBO effectively treats TNBS-induced colitis by increasing the activity of antioxidant enzymes and modulating cytokine profiles.
在大多数情况下，炎症性肠病（IBD）的治疗能够控制症状，但仍有一部分患者的病情没有改善或对治疗反应丧失，因此探索新的治疗策略很重要。高压氧治疗（HBO）可能就是其中之一。本研究旨在评估 HBO 治疗在 IBD 实验模型中的疗效。
将 60 只雄性 BALBc 小鼠分为 6 组。第 1 组通过三硝基苯磺酸（TNBS）+乙醇诱导结肠炎，第 2 组接受 TNBS+乙醇+ HBO 治疗，第 3 组仅接受乙醇处理，第 4 组接受乙醇+ HBO 治疗，第 5 组接受生理盐水处理，第 6 组接受生理盐水+ HBO 治疗。HBO 治疗持续 4 天，随后每天对小鼠进行评估。研究结束时，采集肠道样本进行组织学分析以及抗氧化酶和细胞因子水平的测定。
HBO 显著改善了动物的临床和组织学状况。HBO 治疗增加了所有组的抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性；此外，仅在 TNBS 组和 TNBS+HBO 组之间存在差异，并且治疗促进了促炎细胞因子 IFN-γ、IL-12、IL-17 和 TNF-α的减少以及抗炎细胞因子 IL-4 和 IL-10 的增加，而 IL-13 没有变化。
HBO 通过增加抗氧化酶的活性和调节细胞因子谱，有效治疗 TNBS 诱导的结肠炎。
