恒河猴诱导多能干细胞系（riPSC05）在无饲养层条件下的建立。
Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions.
机构信息
Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA.
Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; UCLA Center for Reproductive Science, Health and Education, Los Angeles, CA 90095, USA.
出版信息
Stem Cell Res. 2023 Dec;73:103241. doi: 10.1016/j.scr.2023.103241. Epub 2023 Nov 4.
We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.
我们使用分离自恒河猴胎儿的成纤维细胞，通过诱导重编程产生并鉴定了恒河猴诱导多能干细胞（iPSC）系。我们将成纤维细胞进行体外培养扩增，然后使用非整合型仙台病毒技术进行重编程。该细胞系被命名为 riPSC05。通过多能性和自我更新标志物的表达、向三个胚层（外胚层、中胚层和内胚层）的体外定向分化、核型分析和 STR 分析，我们确认了 riPSC05 的真实性。
Zotero 插件